Molecular cloning, chromosomal mapping, and functional expression of human brain glutamate receptors

Journal Article · Sat Feb 15 00:00:00 EST 1992 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.89.4.1443· OSTI ID:5445315

Sun, W; Ferrer-Montiel, A V; Schinder, A F; Montal, M ^[1]; McPherson, J P ^[2]; Evans, G A ^[3]

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1. Univ. of California, San Diego, La Jolla (United States)
2. Univ. of California, Irvine (United States)
3. Salk Inst. for Biological Studies, La Jolla, CA (United States)

A full-length cDNA clone encoding a glutamate receptor was isolated from a human brain cDNA library, and the gene product was characterized after expression in Xenopus oocytes. Degenerate PCR primers to conserved regions of published rat brain glutamate receptor sequences amplified a 1-kilobase fragment from a human brain cDNA library. This fragment was used as a probe for subsequent hybridization screening. Two clones were isolated that, based on sequence information, code for different receptors: a 3-kilobase clone, HBGR1, contains a full-length glutamate receptor cDNA highly homologous to the rat brain clone GluR1, and a second clone, HBGR2, contains approximately two-thirds of the coding region of a receptor homologous to rat brain clone GluR2. Southern and PCr analysis of a somatic cell-hybrid panel mapped HBGR1 to human chromosome 5q31.3-33.3 and mapped HBGR2 to chromosome 4q25-34.3. Xenopus oocytes injected with in vitro-synthesized HBGR1 cRNA expressed currents activated by glutamate receptor agonists. These results indicate that clone HBGR1 codes for a glutamate receptor of the kainate subtype cognate to members of the glutamate receptor family from rodent brain.

• OSTI ID: 5445315
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 89:4; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
HUMAN CHROMOSOME 5
GENETIC MAPPING
RECEPTORS
AMINO ACID SEQUENCE
RECOMBINANT DNA
DNA SEQUENCING
AMPHIBIANS
DNA POLYMERASES
GLUTAMIC ACID
MAN
OOCYTES
AMINO ACIDS
ANIMALS
AQUATIC ORGANISMS
CARBOXYLIC ACIDS
CHROMOSOMES
DNA
ENZYMES
GERM CELLS
HUMAN CHROMOSOMES
MAMMALS
MAPPING
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSFERASES
VERTEBRATES
550400* - Genetics