Methyl-coenzyme M, an intermediate in methanogenic dissimilation of C/sub 1/ compounds by Methanosarcina barkeri

Journal Article · Fri Feb 01 00:00:00 EST 1980 · J. Bacteriol.; (United States)

OSTI ID:5439523

Shapiro, S; Wolfe, R S

Extracts of Methanosarcina barkeri possess a specific methyltransferase that catalyzes the transfer of the methyl group of methanol to 2-mercaptoethanesulfonic acid. Over a fourfold range in added 2-mercaptoethanesulfonic acid, the formation of 2-(methylthio)ethanesulfonic acid exhibited a 1:1 ratio to 2-mercaptoethanesulfonic acid added. This reaction required adenosine 5'-triphosphate; a maximal ratio (mole/mol) of 85 methyl groups was transferred per adenosine 5'-triphosphate added. The methyltransferase was found in extracts of methanol-grown cells as well as in extracts of hydrogen-grown cells. In extracts of cells grown on either substrate, 2-(methylthio)ethanesulfonic acid was formed from added methanol or methylamine but not from acetate.

• Research Organization: Univ. of Illinois, Urbana
• OSTI ID: 5439523
• Journal Information: J. Bacteriol.; (United States), Vol. 141:2
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
METHANE
BIOSYNTHESIS
METHANOGENIC BACTERIA
CHEMICAL REACTIONS
METHYL RADICALS
TRANSFERASES
ATP
CELL CULTURES
CHELATING AGENTS
EXPERIMENTAL DATA
GRAPHS
METHANOL
METHYLAMINE
SULFONIC ACID ESTERS
TABLES
THIOLS
ALCOHOLS
ALKANES
ALKYL RADICALS
AMINES
BACTERIA
CRYOGENIC FLUIDS
DATA
DATA FORMS
ENZYMES
ESTERS
FLUIDS
HYDROCARBONS
HYDROXY COMPOUNDS
INFORMATION
MICROORGANISMS
NUCLEOTIDES
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADICALS
SYNTHESIS
140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989)
090122 - Hydrocarbon Fuels- Preparation from Wastes or Biomass- (1976-1989)