Aluminum interaction with human brain tau protein phosphorylation by various kinases

Journal Article · Fri Jan 01 00:00:00 EST 1993 · Journal of Environmental Science and Health, Part B, Pesticides, Food Contaminants, and Agricultural Wastes; (United States)

DOI:https://doi.org/10.1080/03601239309372852· OSTI ID:5438000

El-Sebae, A H; Zeid, M M.A.; Saleh, M A ^[1]; Abdel-Ghany, M E; Shalloway, D ^[2]; Blancato, J ^[3]

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1. Texas Southern Univ., Houston, TX (United States). Environmental Chemistry and Toxicology Lab.
2. Cornell Univ., Ithaca, NY (United States)
3. Environmental Protect Agency, Las Vegas, NV (United States). Environmental Monitoring Systems Lab.

Phosphorylation is an indispensable process for energy and signal transduction in biological systems. AlCl[sub 3] at 10 nM to 10 uM range activated in-vitro [[gamma]-[sup 32]P] ATP phosphorylation of the brain (tau) [Tau] protein in both normal human or E. coli expressed [Tau] forms; in the presence of the kinases P34, PKP, and PKC to a maximum at 1 mM level. AlCl[sub 3] at 100 uM to 500 uM range induced non-enzymatic phosphorylation of [Tau] with [gamma]-ATP, [gamma]-GTP, and [alpha]-GTP, and [alpha]-GTP. AlCl[sub 3] activated histone phosphorylation by P34 in a similar pattern. The hyperphosphorylation of [Tau] with [gamma]-ATP, [gamma]-GTP, and [alpha]-GTP. AlCl[sub 3] activated histone phosphorylation by P34 in a similar pattern. The hyperphosphorylation of [Tau] by Al[sup 3+] was accompanied by molecular shift and mobility retardation in SDS-PAGE. This may demonstrate the mechanism of the longterm neurological effect of Al[sup 3+] in human brain leading to the formation of the neurofibrillary tangles related to Alzeheimer's disease.

• OSTI ID: 5438000
• Journal Information: Journal of Environmental Science and Health, Part B, Pesticides, Food Contaminants, and Agricultural Wastes; (United States), Vol. B28:6; ISSN 0360-1234
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALUMINIUM
BIOLOGICAL EFFECTS
NERVOUS SYSTEM DISEASES
PATHOGENESIS
PROTEINS
PHOSPHORYLATION
MAN
METABOLISM
ANIMALS
CHEMICAL REACTIONS
DISEASES
ELEMENTS
MAMMALS
METALS
ORGANIC COMPOUNDS
PRIMATES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology