Intestinal sucrase inhibitors and bile acid absorption in the rat

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5435738

Walsh, C T; Harnett, K M

Studies were carried out to determine if bile acid absorption is perturbed by the intestinal sucrase inhibitors, Acarbose and BAY m 1099 (1,5-dideoxy-1.5((2-hydroxy-ethyl) imino-)-D-glucitol). The intestinal absorption of taurocholic acid (TA) in male Wistar rats, anesthetized with pentobarbital (50mg/ig, i.p.), was assessed from its excretion rate in bile. In acute studies, 15 cm of distal ileum was perfused in vivo for 70 min with /sup 14/C-TA (0.1 mM, 5 ..mu.. Ci/mmol) in 0.154 M NaCl, 0.01 M phosphate (pH 6.8) and in some studies 20 mM sucrose. From 70-140 min the perfusate was unchanged or contained Acarbose (150, 1500 ..mu..g/ml) or BAY m 1099 (10, 25 ..mu..g/ ml). Neither drug without sucrose altered TA biliary excretion. With sucrose, BAY m 1099 (10 and 25 ..mu.. g/ml) reduced TA excretion by 11 and 22%; no greater effect occurred with 60..mu..g/ml. In subchronic studies rats were fed Acarbose (40 mg/100 g diet) or BAY m 1099 (10, 20, 40 mg/100 g diet) in AIN-76A (50% cornstarch, 15% sucrose) and after 8 wk /sup 14/C-TA (10 mg/kg, 0.08 ..mu..Ci/mg, 3 mg/ml 0.9% NaCl) was injected into the proximal small intestine. Neither drug affected the biliary excretion of TA, measured every 20 min for 4-5 hr. These studies indicate that neither acute nor subchronic regimes of Acarbose or BAY m 1099 affect the intestinal absorption of TA. A possible effect in the presence of sucrose is being explored.

• Research Organization: Boston Univ. School of Medicine, MA
• OSTI ID: 5435738
• Report Number(s): CONF-8604222-; TRN: 86-026658
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BILE ACIDS
INTESTINAL ABSORPTION
ENZYME INHIBITORS
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
EXCRETION
RATS
SACCHAROSE
TRACER TECHNIQUES
ABSORPTION
ANIMALS
CARBOHYDRATES
CARBOXYLIC ACIDS
CLEARANCE
DISACCHARIDES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
OLIGOSACCHARIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
RODENTS
SACCHARIDES
STEROIDS
STEROLS
UPTAKE
VERTEBRATES
550501* - Metabolism- Tracer Techniques