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Title: Quantitative studies of human cardiac metabolism by /sup 31/P rotating-frame NMR

Abstract

The authors have developed /sup 31/P NMR spectroscopic methods to determine quantitatively relative levels of phosphorus-containing metabolites in the human myocardium. They have used localization techniques based on the rotating-frame imaging experiment and carried out the double-surface coil probe. Information is obtained from selected slices by rotating-frame depth selection and from a complete one-dimensional spectroscopic image using phase-modulated rotating-frame imaging. The methods collect biochemical information from metabolites in human heart, and they use the fact that the phosphocreatine/ATP molar ratio in skeletal muscle at rest is higher than that in working heart to demonstrate that localization ha been achieved for each investigation. The phosphocreatine/ATP molar ratio in normal human heart has been measured as 1.55 +/- 0.20 in six subjects using dept selection and as 1.53 +/- 0.25 in four subjects using spectroscopic imaging. Measurement of this ratio is expected to give a useful and reproducible index of myocardial energetics in normal and pathological states.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
John Radcliffe Hospital, Oxford, England
OSTI Identifier:
5435170
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 84:12
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; HEART; DYNAMIC FUNCTION STUDIES; NUCLEAR MAGNETIC RESONANCE; METABOLISM; METABOLITES; MYOCARDIUM; PATIENTS; PHOSPHOCREATINE; PHOSPHORUS 31; AMINO ACIDS; BODY; CARBOXYLIC ACIDS; CARDIOVASCULAR SYSTEM; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MUSCLES; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; RESONANCE; STABLE ISOTOPES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Blackledge, M J, Rajagopalan, B, Oberhaensli, R D, Bolas, N M, Styles, P, and Radda, G K. Quantitative studies of human cardiac metabolism by /sup 31/P rotating-frame NMR. United States: N. p., 1987. Web. doi:10.1073/pnas.84.12.4283.
Blackledge, M J, Rajagopalan, B, Oberhaensli, R D, Bolas, N M, Styles, P, & Radda, G K. Quantitative studies of human cardiac metabolism by /sup 31/P rotating-frame NMR. United States. https://doi.org/10.1073/pnas.84.12.4283
Blackledge, M J, Rajagopalan, B, Oberhaensli, R D, Bolas, N M, Styles, P, and Radda, G K. 1987. "Quantitative studies of human cardiac metabolism by /sup 31/P rotating-frame NMR". United States. https://doi.org/10.1073/pnas.84.12.4283.
@article{osti_5435170,
title = {Quantitative studies of human cardiac metabolism by /sup 31/P rotating-frame NMR},
author = {Blackledge, M J and Rajagopalan, B and Oberhaensli, R D and Bolas, N M and Styles, P and Radda, G K},
abstractNote = {The authors have developed /sup 31/P NMR spectroscopic methods to determine quantitatively relative levels of phosphorus-containing metabolites in the human myocardium. They have used localization techniques based on the rotating-frame imaging experiment and carried out the double-surface coil probe. Information is obtained from selected slices by rotating-frame depth selection and from a complete one-dimensional spectroscopic image using phase-modulated rotating-frame imaging. The methods collect biochemical information from metabolites in human heart, and they use the fact that the phosphocreatine/ATP molar ratio in skeletal muscle at rest is higher than that in working heart to demonstrate that localization ha been achieved for each investigation. The phosphocreatine/ATP molar ratio in normal human heart has been measured as 1.55 +/- 0.20 in six subjects using dept selection and as 1.53 +/- 0.25 in four subjects using spectroscopic imaging. Measurement of this ratio is expected to give a useful and reproducible index of myocardial energetics in normal and pathological states.},
doi = {10.1073/pnas.84.12.4283},
url = {https://www.osti.gov/biblio/5435170}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 84:12,
place = {United States},
year = {Mon Jun 01 00:00:00 EDT 1987},
month = {Mon Jun 01 00:00:00 EDT 1987}
}