Polycythemia in transgenic mice expressing the human erythropoietin gene

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.7.2301· OSTI ID:5434247

Semenza, G L; Traystman, M D; Gearhart, J D; Antonarakis, S E ^[1]

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1. Johns Hopkins Univ. School of Medicine, Baltimore, MD (USA)

Erythropoietin is a glycoprotein hormone that regulates mammalian erythropoiesis. To study the expression of the human erythropoietin gene, EPO, 4 kilobases of DNA encompassing the gene with 0.4 kilobase of 5{prime} flanking sequence and 0.7 kilobase of 3{prime} flanking sequence was microinjected into fertilized mouse eggs. Transgenic mice were generated that are polycythemic, with increased erythrocytic indices in peripheral blood, increased numbers of erythroid precursors in hematopoietic tissue, and increased serum erythropoietin levels. Transgenic homozygotes show a greater degree of polycythemia than do heterozygotes as well as striking extramedullary erythropoiesis. Human erythropoietin RNA was found not only in fetal liver, adult liver, and kidney but also in all other transgenic tissues analyzed. Anemia induced increased human erythropoietin RNA levels in liver but not kidney. These transgenic mice represent a unique model of polycythemia due to increased erythropoietin levels.

• OSTI ID: 5434247
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:7; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ERYTHROPOIETIN
GENE REGULATION
ADULTS
ANEMIAS
BLOOD COUNT
BLOOD PLATELETS
FETUSES
GENES
GLYCOPROTEINS
HEMATOLOGY
KIDNEYS
LIVER
MAN
MESSENGER-RNA
MICE
PHOSPHORUS 32
AGE GROUPS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
DISEASES
GLANDS
HEMIC DISEASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
MEDICINE
MITOGENS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
RODENTS
SYMPTOMS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques