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Title: Unique properties of cd-binding peptides induced in fission yeast, Schizosaccharomyces pombe

Abstract

Metallothioneins, a class of low molecular weight cysteine-rich proteins that bind heavy metal ions, have been found in various eucaryotic organisms. When fission yeasts are grown in the presence of high concentration of CdCl/sub 2/, large amounts of Cd-binding peptides (Cd-BP1 and Cd-BP2) are synthesized. While Cd-BP2 shows similarities to mammalian Cd-thioneins in UV and CD spectra, Cd-BP1has a characteristic shoulder at 265 nm in the UV absorption spectrum and shows two marked Cotton bands at 257 nm (negative) and 275 nm (positive). These characteristics of Cd-BP1 are not found in the other Cd-thioneins. The UV and CD spectra differences between reconstituted and native Cd-BP1 suggest the requirement for some additional molecular architecture including another peptide-Cd/sup 2 +/ interaction. Induction of cadystin synthesis is almost exclusive for Cd, but an exception is a small amount of cadystin also induced by the higher concentration of CuCl/sub 2/ (2.5 mM). The UV spectrum of the natural Cu-cadystin complex was similar to that of Cd-BP1. On the basis of these findings the models for Cd-BP1 and Cd-BP2 are proposed.

Authors:
; ;
Publication Date:
Research Org.:
Institute for Developmental Research, Aichi, Japan
OSTI Identifier:
5431660
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environ. Health Perspect.; (United States); Journal Volume: 65
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 59 BASIC BIOLOGICAL SCIENCES; CADMIUM CHLORIDES; BIOLOGICAL EFFECTS; METALLOPROTEINS; BIOCHEMISTRY; CHEMICAL PROPERTIES; SACCHAROMYCES; ULTRAVIOLET SPECTRA; CADMIUM COMPOUNDS; CADMIUM HALIDES; CHEMISTRY; CHLORIDES; CHLORINE COMPOUNDS; FUNGI; HALIDES; HALOGEN COMPOUNDS; MICROORGANISMS; ORGANIC COMPOUNDS; PLANTS; PROTEINS; SPECTRA; YEASTS; 560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987); 550200 - Biochemistry

Citation Formats

Hayashi, Y., Nakagawa, C.W., and Murasugi, A.. Unique properties of cd-binding peptides induced in fission yeast, Schizosaccharomyces pombe. United States: N. p., 1986. Web. doi:10.1289/ehp.866513.
Hayashi, Y., Nakagawa, C.W., & Murasugi, A.. Unique properties of cd-binding peptides induced in fission yeast, Schizosaccharomyces pombe. United States. doi:10.1289/ehp.866513.
Hayashi, Y., Nakagawa, C.W., and Murasugi, A.. Sat . "Unique properties of cd-binding peptides induced in fission yeast, Schizosaccharomyces pombe". United States. doi:10.1289/ehp.866513.
@article{osti_5431660,
title = {Unique properties of cd-binding peptides induced in fission yeast, Schizosaccharomyces pombe},
author = {Hayashi, Y. and Nakagawa, C.W. and Murasugi, A.},
abstractNote = {Metallothioneins, a class of low molecular weight cysteine-rich proteins that bind heavy metal ions, have been found in various eucaryotic organisms. When fission yeasts are grown in the presence of high concentration of CdCl/sub 2/, large amounts of Cd-binding peptides (Cd-BP1 and Cd-BP2) are synthesized. While Cd-BP2 shows similarities to mammalian Cd-thioneins in UV and CD spectra, Cd-BP1has a characteristic shoulder at 265 nm in the UV absorption spectrum and shows two marked Cotton bands at 257 nm (negative) and 275 nm (positive). These characteristics of Cd-BP1 are not found in the other Cd-thioneins. The UV and CD spectra differences between reconstituted and native Cd-BP1 suggest the requirement for some additional molecular architecture including another peptide-Cd/sup 2 +/ interaction. Induction of cadystin synthesis is almost exclusive for Cd, but an exception is a small amount of cadystin also induced by the higher concentration of CuCl/sub 2/ (2.5 mM). The UV spectrum of the natural Cu-cadystin complex was similar to that of Cd-BP1. On the basis of these findings the models for Cd-BP1 and Cd-BP2 are proposed.},
doi = {10.1289/ehp.866513},
journal = {Environ. Health Perspect.; (United States)},
number = ,
volume = 65,
place = {United States},
year = {Sat Mar 01 00:00:00 EST 1986},
month = {Sat Mar 01 00:00:00 EST 1986}
}