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Title: Offspring of female brindled mice fed a copper-deficient diet develop anemia

Abstract

Female C57BL mice heterozygous for the brindled gene (Mo/sup br/+/) were mated to normal males and fed a purified diet low in copper(-Cu)(0.5 ppm), modified AIN-76A, throughout gestation and lactation to investigate copper-dependent anemia. Half the females were given deionized water and half copper-supplemented water (20 ppm Cu as CuSO/sub 4/). Four groups of 12 day old male offspring were compared (Dunn-Bonferroni t-test ..cap alpha.. = 0.05): I = +Cu(Mo/sup +/y/), II = -Cu(Mo/sup +/y/), III = +Cu(Mo/sup br/y/), IV = -Cu(Mo/sup br/y/). Offspring in I and III were equivalent to those in earlier studies when dams were fed Mouse-Chow. Liver Cu levels in I are below normal. Compared to III the mice in IV were smaller and had lower hematocrits, however, liver Cu and Fe levels were similar. Liver superoxide dismutase (SOD) activity was lower in IV compared to III. Compared to II, mice in IV were smaller and thus had higher hematocrits and liver Fe. Liver Cu and SOD activities were similar but plasma ceruloplasmin activities were higher in IV. Offspring of brindled female mice exhibit lower liver copper levels compared to offspring from normal females. This is true whether dams are fed a nonpurified diet or themore » -Cu diet with or without a Cu supplement in the water. Thus, all offspring of brindled dams have abnormal copper homeostasis during perinatal development. When copper is limiting brindled mice will develop anemia.« less

Authors:
;
Publication Date:
Research Org.:
Univ. of Minnesota, Duluth
OSTI Identifier:
5431139
Alternate Identifier(s):
OSTI ID: 5431139
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA
Resource Type:
Conference
Resource Relation:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States); Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; COPPER; BIOLOGICAL EFFECTS; NUTRITIONAL DEFICIENCY; SUPEROXIDE DISMUTASE; ENZYME ACTIVITY; ANEMIAS; BLOOD COUNT; CERULOPLASMIN; DIET; LACTATION; LIVER; MICE; PREGNANCY; ANIMALS; BODY; COMPLEXES; COPPER COMPLEXES; DIGESTIVE SYSTEM; DISEASES; ELEMENTS; ENZYMES; GLANDS; GLOBULINS; GLOBULINS-ALPHA; HEMIC DISEASES; MAMMALS; METALLOPROTEINS; METALS; ORGANIC COMPOUNDS; ORGANS; OXIDOREDUCTASES; PROTEINS; RODENTS; SYMPTOMS; TRANSITION ELEMENT COMPLEXES; TRANSITION ELEMENTS; VERTEBRATES 560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)

Citation Formats

Prohaska, J.R., and Bailey, W.R. Offspring of female brindled mice fed a copper-deficient diet develop anemia. United States: N. p., 1986. Web.
Prohaska, J.R., & Bailey, W.R. Offspring of female brindled mice fed a copper-deficient diet develop anemia. United States.
Prohaska, J.R., and Bailey, W.R. Sat . "Offspring of female brindled mice fed a copper-deficient diet develop anemia". United States. doi:.
@article{osti_5431139,
title = {Offspring of female brindled mice fed a copper-deficient diet develop anemia},
author = {Prohaska, J.R. and Bailey, W.R.},
abstractNote = {Female C57BL mice heterozygous for the brindled gene (Mo/sup br/+/) were mated to normal males and fed a purified diet low in copper(-Cu)(0.5 ppm), modified AIN-76A, throughout gestation and lactation to investigate copper-dependent anemia. Half the females were given deionized water and half copper-supplemented water (20 ppm Cu as CuSO/sub 4/). Four groups of 12 day old male offspring were compared (Dunn-Bonferroni t-test ..cap alpha.. = 0.05): I = +Cu(Mo/sup +/y/), II = -Cu(Mo/sup +/y/), III = +Cu(Mo/sup br/y/), IV = -Cu(Mo/sup br/y/). Offspring in I and III were equivalent to those in earlier studies when dams were fed Mouse-Chow. Liver Cu levels in I are below normal. Compared to III the mice in IV were smaller and had lower hematocrits, however, liver Cu and Fe levels were similar. Liver superoxide dismutase (SOD) activity was lower in IV compared to III. Compared to II, mice in IV were smaller and thus had higher hematocrits and liver Fe. Liver Cu and SOD activities were similar but plasma ceruloplasmin activities were higher in IV. Offspring of brindled female mice exhibit lower liver copper levels compared to offspring from normal females. This is true whether dams are fed a nonpurified diet or the -Cu diet with or without a Cu supplement in the water. Thus, all offspring of brindled dams have abnormal copper homeostasis during perinatal development. When copper is limiting brindled mice will develop anemia.},
doi = {},
journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:3,
place = {United States},
year = {Sat Mar 01 00:00:00 EST 1986},
month = {Sat Mar 01 00:00:00 EST 1986}
}

Conference:
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