DLH1 is a functional Candida albicans homologue of the meiosis-specific gene DMC1
- Massachusetts Institute of Technology, Cambridge, MA (United States)
DMC1/LIM15 homologue 1 (DLH1), a gene related to meiosis-specific genes, has been isolated from Candida albicans, a fungus thought not to undergo meiosis. The deduced protein sequence of DLH1 contains 74% amino acid identity with Dmc1p from Saccharomyces cerevisiae and 63% with Lim15p from the plant Lilium longiflorum, meiosis-specific homologous of Escherichia coli RecA. Candida DLH1 complements a dmc1/dmc1 null mutant in S. cerevisiae. High copy expression of DLH1 restores both sporulation and meiotic recombination to a Saccharomyces dmc1/{Delta}/dmc1{Delta} strain. Unlike the DMC1 gene, which is transcribed only in meiotic cells, the heterologous Candida DLH1 gene is transcribed in both vegetative and meiotic cells of S. cerevisiae. Transcription of DLH1 is not detected or induced in C. albicans under conditions that induce DMC1 and meiosis in S. cerevisiae. The presence of an intact homologue of a meiosis-specific gene in C. albicans raises the possibility that this organism has a cryptic meiotic pathway. 25 refs., 6 figs., 3 tabs.
- OSTI ID:
- 543015
- Journal Information:
- Genetics, Vol. 143, Issue 2; Other Information: PBD: Jun 1996
- Country of Publication:
- United States
- Language:
- English
Similar Records
Gene Annotation and Drug Target Discovery in Candida albicans with a Tagged Transposon Mutant Collection
Directed mutagenesis in Candida albicans: one-step gene disruption to isolate ura3 mutants