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Title: Development of a mutant strain of Bacillus polymyxa showing enhanced production of 2,3-butanediol

Abstract

2,3-Butanediol is a feedstock chemical of potential industrial importance. It can serve as a monomer for many polymers of consumer interest that are currently supplied by the fossil fuel industry. Bacillus polymyxa can grow on inexpensive waste products of the food-processing industry and produce this glycol. This paper describes a mutant strain of B. polymyxa which displays constitutive production of catabolic ..cap alpha..-acetolactate synthase, an enzyme in the 2,3-butanediol pathway which is normally produced only in the late log or stationary phase of growth. The mutant was obtained by treating the wild type with nitrosoguanidine and subjecting it to a penicillin counterselection procedure. One of the selected mutant strains produced four times as much of the glycol as the wild type and utilized approximately 25% of the energy source, compared with essentially complete utilization of the energy source by the wild type. Studies are under way to optimize the production of the glycol by the mutant.

Authors:
;
Publication Date:
Research Org.:
Univ. of Illinois, Urbana
OSTI Identifier:
5429290
Resource Type:
Journal Article
Journal Name:
Appl. Environ. Microbiol.; (United States)
Additional Journal Information:
Journal Volume: 54:1
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; BACILLUS; GENETIC ENGINEERING; BUTANEDIOLS; BIOSYNTHESIS; INDUSTRIAL WASTES; FERMENTATION; BIOTECHNOLOGY; MUTAGENESIS; MUTANTS; NITROSO COMPOUNDS; OPTIMIZATION; ALCOHOLS; BACTERIA; BIOCONVERSION; GLYCOLS; HYDROXY COMPOUNDS; MICROORGANISMS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; SYNTHESIS; WASTES; 090222* - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989); 140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 550700 - Microbiology

Citation Formats

Mallonee, D H, and Speckman, R A. Development of a mutant strain of Bacillus polymyxa showing enhanced production of 2,3-butanediol. United States: N. p., 1988. Web.
Mallonee, D H, & Speckman, R A. Development of a mutant strain of Bacillus polymyxa showing enhanced production of 2,3-butanediol. United States.
Mallonee, D H, and Speckman, R A. 1988. "Development of a mutant strain of Bacillus polymyxa showing enhanced production of 2,3-butanediol". United States.
@article{osti_5429290,
title = {Development of a mutant strain of Bacillus polymyxa showing enhanced production of 2,3-butanediol},
author = {Mallonee, D H and Speckman, R A},
abstractNote = {2,3-Butanediol is a feedstock chemical of potential industrial importance. It can serve as a monomer for many polymers of consumer interest that are currently supplied by the fossil fuel industry. Bacillus polymyxa can grow on inexpensive waste products of the food-processing industry and produce this glycol. This paper describes a mutant strain of B. polymyxa which displays constitutive production of catabolic ..cap alpha..-acetolactate synthase, an enzyme in the 2,3-butanediol pathway which is normally produced only in the late log or stationary phase of growth. The mutant was obtained by treating the wild type with nitrosoguanidine and subjecting it to a penicillin counterselection procedure. One of the selected mutant strains produced four times as much of the glycol as the wild type and utilized approximately 25% of the energy source, compared with essentially complete utilization of the energy source by the wild type. Studies are under way to optimize the production of the glycol by the mutant.},
doi = {},
url = {https://www.osti.gov/biblio/5429290}, journal = {Appl. Environ. Microbiol.; (United States)},
number = ,
volume = 54:1,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}