Enzymological studies of one-carbon reactions in the pathway of acetate utilization by methanogenic bacteria: Annual technical report for the period 6/1/87 to 1/31/88
The general pathway of acetate conversion to methane in Methanosarcina thermophila is known and several of the enzymes have been characterized. AcetylCoA is cleaved by a enzyme complex with carbon monoxide dehydrogenases activity. The methyl group is transferred to Coenzyme M and the carbonyl to the nickel site in the complex. Methylcoenzyme M is reductively demethylated to methane with electrons derived from the oxidation of the bound carbonyl. A ferredoxin is the immediate electron acceptor of electrons leaving the complex. Membrane components are implicated in electron transport from the ferredoxin to methylcoenzyme m. A corrinoid protein in the complex is thought to catalyze methyl transfer, electron transfer, or both. All of the above proteins have been purified except the corrinoid protein. Our laboratory is investigating the catalytic mechanism of the purified proteins. Biochemical characterizations of all proteins will include amino acid analysis and N-terminal sequencing, kinetic parameters, physical and other properties. 2 tabs.
- Research Organization:
- Virginia Polytechnic Inst., Blacksburg (USA)
- DOE Contract Number:
- FG05-87ER13730
- OSTI ID:
- 5429132
- Report Number(s):
- DOE/ER/13730-1; ON: DE88006539
- Country of Publication:
- United States
- Language:
- English
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Enzymological studies of one-carbon reactions in the pathway of acetate utilization by methanogenic bacteria
Enzymological studies of one-carbon reactions in the pathway of acetate utilization by methanogenic bacteria: Annual technical report for the period February 1, 1988--January 31, 1989
Related Subjects
59 BASIC BIOLOGICAL SCIENCES
ACETATES
CATABOLISM
FERREDOXIN
FRACTIONATION
METHANE
BIOSYNTHESIS
METHANOGENIC BACTERIA
ENZYME ACTIVITY
CARBON MONOXIDE
CYTOCHROMES
OXIDOREDUCTASES
PROGRESS REPORT
ALKANES
BACTERIA
CARBON COMPOUNDS
CARBON OXIDES
CARBOXYLIC ACID SALTS
CHALCOGENIDES
DOCUMENT TYPES
ENZYMES
HYDROCARBONS
METABOLISM
METALLOPROTEINS
MICROORGANISMS
ORGANIC COMPOUNDS
OXIDES
OXYGEN COMPOUNDS
PIGMENTS
PROTEINS
SEPARATION PROCESSES
SYNTHESIS
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