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Title: Scattered electrons in microscopy and microanalysis

Abstract

The use of scattered electrons alone for direct imaging of biological specimens makes it possible to obtain structural information at atomic and near-atomic spatial resolutions of 0.3 to 0.5 nanometer. While this is not as good as the resolution possible with x-ray crystallography, such an approach provides structural information rapidly on individual macromolecules that have not been, and possibly cannot be, crystallized. Analysis of the spectrum of energies of scattered electrons and imaging of the latter with characteristic energy bands within the spectrum produces a powerful new technique of atomic microanalysis. This technique, which has a spatial resolution of about 0.5 nanometer and a minimum detection sensitivity of about 50 atoms of phosphorus, is especially useful for light atom analysis and appears to have applications in molecular biology, cell biology, histology, pathology, botany, and many other fields.

Authors:
Publication Date:
Research Org.:
Department of Medical Biophysics, University of Toronto, Ontario, Canada
OSTI Identifier:
5428237
Resource Type:
Journal Article
Resource Relation:
Journal Name: Science (Washington, D.C.); (United States); Journal Volume: 215:4532
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; ELECTRON MICROPROBE ANALYSIS; SPATIAL RESOLUTION; HISTOLOGY; MOLECULAR BIOLOGY; MOLECULAR STRUCTURE; PATHOLOGY; STRUCTURAL CHEMICAL ANALYSIS; CHEMICAL ANALYSIS; MICROANALYSIS; RESOLUTION; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 550301 - Cytology- Tracer Techniques; 550901 - Pathology- Tracer Techniques

Citation Formats

Ottensmeyer, F.P. Scattered electrons in microscopy and microanalysis. United States: N. p., 1982. Web. doi:10.1126/science.7054874.
Ottensmeyer, F.P. Scattered electrons in microscopy and microanalysis. United States. doi:10.1126/science.7054874.
Ottensmeyer, F.P. Fri . "Scattered electrons in microscopy and microanalysis". United States. doi:10.1126/science.7054874.
@article{osti_5428237,
title = {Scattered electrons in microscopy and microanalysis},
author = {Ottensmeyer, F.P.},
abstractNote = {The use of scattered electrons alone for direct imaging of biological specimens makes it possible to obtain structural information at atomic and near-atomic spatial resolutions of 0.3 to 0.5 nanometer. While this is not as good as the resolution possible with x-ray crystallography, such an approach provides structural information rapidly on individual macromolecules that have not been, and possibly cannot be, crystallized. Analysis of the spectrum of energies of scattered electrons and imaging of the latter with characteristic energy bands within the spectrum produces a powerful new technique of atomic microanalysis. This technique, which has a spatial resolution of about 0.5 nanometer and a minimum detection sensitivity of about 50 atoms of phosphorus, is especially useful for light atom analysis and appears to have applications in molecular biology, cell biology, histology, pathology, botany, and many other fields.},
doi = {10.1126/science.7054874},
journal = {Science (Washington, D.C.); (United States)},
number = ,
volume = 215:4532,
place = {United States},
year = {Fri Jan 29 00:00:00 EST 1982},
month = {Fri Jan 29 00:00:00 EST 1982}
}