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Title: Modulation of acetylcholine release from rat striatal slices by the GABA/benzodiazepine receptor complex

Abstract

GABA, THIP and muscimol enhance spontaneous and inhibit electrically induced release of tritium labelled compounds from rat striatal slices which have been pre-labelled with /sup 3/H-choline. Baclofen is inactive in this model. Muscimol can inhibit electrically induced release of tritiated material by approximately 75% with half maximal effects at 2 ..mu..M. The response to muscimol can be blocked by the GABA antagonists bicuculline methobromide, picrotoxin, anisatin, R 5135 and CPTBO (cyclopentylbicyclophosphate). Drugs which act on the benzodiazepine receptor (BR) require the presence of muscimol to be effective and they modulate the effects of muscimol in a bidirectional manner. Thus BR agonists enhance and inverse BR agonists attenuate the inhibitory effects of muscimol on electrically induced release. Ro15-1788, a BR antagonist, does not modulate the inhibitory effects of muscimol but antagonizes the actions of clonazepam, a BR agonist, and of DMCM, an inverse BR agonist. These results demonstrate that a GABA/benzodiazepine receptor complex can modulate acetylcholine release from rat striatal slices in vitro. 24 references, 3 figures, 5 table.

Authors:
;
Publication Date:
Research Org.:
Sandoz Ltd., Basle, Switzerland
OSTI Identifier:
5416281
Resource Type:
Journal Article
Journal Name:
Life Sci.; (United States)
Additional Journal Information:
Journal Volume: 36:5
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AMINOBUTYRIC ACID; RECEPTORS; FUNCTIONAL MODELS; TRANQUILIZERS; ACETYLCHOLINE; BIOCHEMISTRY; CHOLINE; IN VITRO; MUSCLES; RATS; TRITIUM COMPOUNDS; ALCOHOLS; AMINES; AMINO ACIDS; AMMONIUM COMPOUNDS; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM AGENTS; CHEMISTRY; DRUGS; ESTERS; HYDROXY COMPOUNDS; LABELLED COMPOUNDS; LIPOTROPIC FACTORS; MAMMALS; NEUROREGULATORS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PARASYMPATHOMIMETICS; PSYCHOTROPIC DRUGS; QUATERNARY COMPOUNDS; RODENTS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Supavilai, P., and Karobath, M. Modulation of acetylcholine release from rat striatal slices by the GABA/benzodiazepine receptor complex. United States: N. p., 1985. Web. doi:10.1016/0024-3205(85)90253-X.
Supavilai, P., & Karobath, M. Modulation of acetylcholine release from rat striatal slices by the GABA/benzodiazepine receptor complex. United States. doi:10.1016/0024-3205(85)90253-X.
Supavilai, P., and Karobath, M. Mon . "Modulation of acetylcholine release from rat striatal slices by the GABA/benzodiazepine receptor complex". United States. doi:10.1016/0024-3205(85)90253-X.
@article{osti_5416281,
title = {Modulation of acetylcholine release from rat striatal slices by the GABA/benzodiazepine receptor complex},
author = {Supavilai, P. and Karobath, M.},
abstractNote = {GABA, THIP and muscimol enhance spontaneous and inhibit electrically induced release of tritium labelled compounds from rat striatal slices which have been pre-labelled with /sup 3/H-choline. Baclofen is inactive in this model. Muscimol can inhibit electrically induced release of tritiated material by approximately 75% with half maximal effects at 2 ..mu..M. The response to muscimol can be blocked by the GABA antagonists bicuculline methobromide, picrotoxin, anisatin, R 5135 and CPTBO (cyclopentylbicyclophosphate). Drugs which act on the benzodiazepine receptor (BR) require the presence of muscimol to be effective and they modulate the effects of muscimol in a bidirectional manner. Thus BR agonists enhance and inverse BR agonists attenuate the inhibitory effects of muscimol on electrically induced release. Ro15-1788, a BR antagonist, does not modulate the inhibitory effects of muscimol but antagonizes the actions of clonazepam, a BR agonist, and of DMCM, an inverse BR agonist. These results demonstrate that a GABA/benzodiazepine receptor complex can modulate acetylcholine release from rat striatal slices in vitro. 24 references, 3 figures, 5 table.},
doi = {10.1016/0024-3205(85)90253-X},
journal = {Life Sci.; (United States)},
number = ,
volume = 36:5,
place = {United States},
year = {1985},
month = {2}
}