Influence of histamine of precursors of granulocytic leukocytes in murine bone marrow
The effect of histamine on granulocytic progenitor cells in murine bone marrow was studied in vitro. When bone marrow cells were cultured for three days with the drug, 10/sup -8/ M to 10/sup -5/ M of histamine stimulated differentiation and proliferation of myeloid precursor cells. Subsequently, the number of descendant cells, such as metamyelocytes and neutrophils, increased dose-dependently. Co-existence of equimolar H/sub 2/ blockers such as cimetidine and ranitidine completely suppressed this effect of histamine, though this was not the case with an H/sub 1/ blocker/histamine combination. Significant increase in /sup 3/H-thymidine incorporation was observed almost exclusively in myeloblasts, promyelocytes and myelocytes after exposure to histamine at concentrations higher than 10/sup -8/ M. Also, selective incorporation of /sup 3/H-histamine into bone marrow cells was observed in myeloblasts and promyelocytes, but histamine incorporation was not influenced by the presence of either of histamine agonists of antagonists. While histamine, via H/sub 2/ receptors, selectively increased the number of granulocytic colony forming units in culture (CFU-C), it had no such effect on macrophage colonies. 22 references, 5 figures, 4 tables.
- Research Organization:
- Okayama Univ., Japan
- OSTI ID:
- 5412192
- Journal Information:
- Life Sci.; (United States), Vol. 42:9
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HISTAMINE
BIOLOGICAL EFFECTS
LEUKOCYTES
CELL PROLIFERATION
BONE MARROW CELLS
CELL CULTURES
DOSE-RESPONSE RELATIONSHIPS
IN VITRO
MICE
PRECURSOR
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
ANIMAL CELLS
ANIMALS
AZINES
AZOLES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
HETEROCYCLIC COMPOUNDS
IMIDAZOLES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIMIDINES
RIBOSIDES
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques