skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Evidence for a K/sup +/-H/sup +/ exchanger in rabbit renal cortical brush-border membrane vesicles

Abstract

An outward-directed H/sup +/ gradient stimulated /sup 86/Rb/sup +/ uptake in brush-border membrane vesicles isolated from rabbit renal cortex. This H/sup +/ gradient-stimulated /sup 86/Rb/sup +/ uptake was inhibited by unlabeled K/sup +/ (IC/sub 50/ = 7.5 mM). The carrier system responsible for this uptake is different from the Na/sup +/-H/sup +/ exchanger, because inhibitors of the Na/sup +/-H/sup +/ exchanger did not affect or affected minimally the /sup 86/Rb/sup +/ uptake whereas these compounds caused 60-85% inhibition of the /sup 22/Na/sup +/ uptake. Moreover, /sup 86/Rb/sup +/ uptake was preferentially inhibited by unlabeled K/sup +/ and Rb/sup +/ whereas /sup 22/Na/sup +/ uptake was preferentially inhibited by Na/sup +/ and Li/sup +/. Furosemide, an inhibitor of the Na/sup +/-K/sup +/-2Cl/sup -/ cotransporter and Ca/sup 2 +/ and Ba/sup 2 +/, modulators of the K/sup +/-channel did not affect /sup 86/Rb uptake in the renal brush-border membrane vesicles, indicating that these transport mechanisms were also not responsible for the observed /sup 86/Rb/sup +/ uptake. These results provide evidence for the presence of a distinct K/sup +/-H/sup +/ exchanger in renal cortical brush-border membrane vesicles.

Authors:
; ; ;
Publication Date:
Research Org.:
Medical College of Georgia, Augusta (USA)
OSTI Identifier:
5409542
Resource Type:
Journal Article
Journal Name:
Ga. J. Sci.; (United States)
Additional Journal Information:
Journal Volume: 45:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; RUBIDIUM COMPOUNDS; MEMBRANE TRANSPORT; SODIUM COMPOUNDS; CATIONS; CELL MEMBRANES; HYDROGEN IONS; INHIBITION; KIDNEYS; POTASSIUM COMPOUNDS; RABBITS; RUBIDIUM 86; SODIUM 22; TRACER TECHNIQUES; ALKALI METAL COMPOUNDS; ALKALI METAL ISOTOPES; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BETA-PLUS DECAY RADIOISOTOPES; BODY; CELL CONSTITUENTS; CHARGED PARTICLES; DAYS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IONS; ISOMERIC TRANSITION ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; LIGHT NUCLEI; MAMMALS; MEMBRANES; MINUTES LIVING RADIOISOTOPES; NUCLEI; ODD-ODD NUCLEI; ORGANS; RADIOISOTOPES; RUBIDIUM ISOTOPES; SODIUM ISOTOPES; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Miyamoto, Y, Tiruppathi, C, Ganapathy, V, and Leibach, F H. Evidence for a K/sup +/-H/sup +/ exchanger in rabbit renal cortical brush-border membrane vesicles. United States: N. p., 1987. Web.
Miyamoto, Y, Tiruppathi, C, Ganapathy, V, & Leibach, F H. Evidence for a K/sup +/-H/sup +/ exchanger in rabbit renal cortical brush-border membrane vesicles. United States.
Miyamoto, Y, Tiruppathi, C, Ganapathy, V, and Leibach, F H. 1987. "Evidence for a K/sup +/-H/sup +/ exchanger in rabbit renal cortical brush-border membrane vesicles". United States.
@article{osti_5409542,
title = {Evidence for a K/sup +/-H/sup +/ exchanger in rabbit renal cortical brush-border membrane vesicles},
author = {Miyamoto, Y and Tiruppathi, C and Ganapathy, V and Leibach, F H},
abstractNote = {An outward-directed H/sup +/ gradient stimulated /sup 86/Rb/sup +/ uptake in brush-border membrane vesicles isolated from rabbit renal cortex. This H/sup +/ gradient-stimulated /sup 86/Rb/sup +/ uptake was inhibited by unlabeled K/sup +/ (IC/sub 50/ = 7.5 mM). The carrier system responsible for this uptake is different from the Na/sup +/-H/sup +/ exchanger, because inhibitors of the Na/sup +/-H/sup +/ exchanger did not affect or affected minimally the /sup 86/Rb/sup +/ uptake whereas these compounds caused 60-85% inhibition of the /sup 22/Na/sup +/ uptake. Moreover, /sup 86/Rb/sup +/ uptake was preferentially inhibited by unlabeled K/sup +/ and Rb/sup +/ whereas /sup 22/Na/sup +/ uptake was preferentially inhibited by Na/sup +/ and Li/sup +/. Furosemide, an inhibitor of the Na/sup +/-K/sup +/-2Cl/sup -/ cotransporter and Ca/sup 2 +/ and Ba/sup 2 +/, modulators of the K/sup +/-channel did not affect /sup 86/Rb uptake in the renal brush-border membrane vesicles, indicating that these transport mechanisms were also not responsible for the observed /sup 86/Rb/sup +/ uptake. These results provide evidence for the presence of a distinct K/sup +/-H/sup +/ exchanger in renal cortical brush-border membrane vesicles.},
doi = {},
url = {https://www.osti.gov/biblio/5409542}, journal = {Ga. J. Sci.; (United States)},
number = ,
volume = 45:2,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1987},
month = {Thu Jan 01 00:00:00 EST 1987}
}