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Title: Liquid chromatography/time-of-flight mass spectrometry with high-speed integrated transient recording

Abstract

High-performance liquid chromatography (HPLC) has been interfaced to a time-of-flight mass spectrometer. The interface is a continuous flow probe and ions are desorbed from the liquid matrix by energetic ion bombardment. Pulsed and delayed ion extraction from the source permits the use of broad ionization times, results in the production of analog signals in each time-of-flight cycle, and provides both energy and spatial focusing. A high-speed integrated transient recording system has been developed and is also reported. This instrument is the prototype for development of a high-speed, high-mass range LC detector with high duty cycle. Its performance is demonstrated for the separation of several mixtures of small peptides.

Authors:
; ;  [1];  [2];  [3]
  1. (Johns Hopkins Univ., Baltimore (USA))
  2. (Univ. of Maryland, Baltimore (USA))
  3. (Kratos Analytical, Manchester (England))
Publication Date:
OSTI Identifier:
5406942
Resource Type:
Journal Article
Journal Name:
Analytical Chemistry (Washington); (United States)
Additional Journal Information:
Journal Volume: 62:13; Journal ID: ISSN 0003-2700
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; LIQUID COLUMN CHROMATOGRAPHY; MEASURING INSTRUMENTS; MASS SPECTROSCOPY; DATA ACQUISITION SYSTEMS; MEASURING METHODS; PEPTIDES; TIME-OF-FLIGHT MASS SPECTROMETERS; CHROMATOGRAPHY; DYNAMIC MASS SPECTROMETERS; MASS SPECTROMETERS; ORGANIC COMPOUNDS; PROTEINS; SEPARATION PROCESSES; SPECTROMETERS; SPECTROSCOPY; TIME-OF-FLIGHT SPECTROMETERS; 400201* - Chemical & Physicochemical Properties

Citation Formats

Emary, W.B., Lys, I., Cotter, R.J., Simpson, R., and Hoffman, A. Liquid chromatography/time-of-flight mass spectrometry with high-speed integrated transient recording. United States: N. p., 1990. Web. doi:10.1021/ac00212a022.
Emary, W.B., Lys, I., Cotter, R.J., Simpson, R., & Hoffman, A. Liquid chromatography/time-of-flight mass spectrometry with high-speed integrated transient recording. United States. doi:10.1021/ac00212a022.
Emary, W.B., Lys, I., Cotter, R.J., Simpson, R., and Hoffman, A. Sun . "Liquid chromatography/time-of-flight mass spectrometry with high-speed integrated transient recording". United States. doi:10.1021/ac00212a022.
@article{osti_5406942,
title = {Liquid chromatography/time-of-flight mass spectrometry with high-speed integrated transient recording},
author = {Emary, W.B. and Lys, I. and Cotter, R.J. and Simpson, R. and Hoffman, A.},
abstractNote = {High-performance liquid chromatography (HPLC) has been interfaced to a time-of-flight mass spectrometer. The interface is a continuous flow probe and ions are desorbed from the liquid matrix by energetic ion bombardment. Pulsed and delayed ion extraction from the source permits the use of broad ionization times, results in the production of analog signals in each time-of-flight cycle, and provides both energy and spatial focusing. A high-speed integrated transient recording system has been developed and is also reported. This instrument is the prototype for development of a high-speed, high-mass range LC detector with high duty cycle. Its performance is demonstrated for the separation of several mixtures of small peptides.},
doi = {10.1021/ac00212a022},
journal = {Analytical Chemistry (Washington); (United States)},
issn = {0003-2700},
number = ,
volume = 62:13,
place = {United States},
year = {1990},
month = {7}
}