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Title: Passage of albumin from the middle ear to the inner ear in otitis media in the chinchilla

Abstract

A study of the permeability of the middle ear-inner ear interface for macromolecules was carried out in chinchillas with open and obstructed eustachian tubes utilizing tritiated human serum albumin and immunoelectrophoresis. Tritiated albumin was placed in the round window niche area or normal animals and animals in which the eustachian tubes had been obstructed for 24 hours or 14 days. The tritiated albumin was allowed to remain in the middle ear cavity for 24 hours, Samples of middle ear effusion, perilymph, blood and cerebrospinal fluid were collected and measured for radioactivity. Radioactivity was demonstrated in the perilymph. Samples of middle ear effusions and perilymph were also studied by immunoelectrophoresis with goat antihuman albumin. Albumin placed in the round window niche of an experimental animal could be recovered unchanged in the perilymph. The results suggest a pathophysiologic explanation for the association of otitis media and sensorineural hearing loss or endolymphatic hydrops.

Authors:
; ; ; ; ; ;
Publication Date:
OSTI Identifier:
5406850
Resource Type:
Journal Article
Journal Name:
Am. J. Otolaryngol.; (United States)
Additional Journal Information:
Journal Volume: 2:3
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; AUDITORY ORGANS; PERMEABILITY; TRITIUM COMPOUNDS; ISOTOPE APPLICATIONS; ALBUMINS; BLOOD; CEREBROSPINAL FLUID; LYMPH; BIOLOGICAL MATERIALS; BODY; BODY FLUIDS; LABELLED COMPOUNDS; MATERIALS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; SENSE ORGANS; 550201* - Biochemistry- Tracer Techniques; 551001 - Physiological Systems- Tracer Techniques

Citation Formats

Goldberg, B., Goycoolea, M.V., Schleivert, P.M., Shea, D., Schachern, P., Paparella, M.M., and Carpenter, A.M. Passage of albumin from the middle ear to the inner ear in otitis media in the chinchilla. United States: N. p., 1981. Web. doi:10.1016/S0196-0709(81)80017-8.
Goldberg, B., Goycoolea, M.V., Schleivert, P.M., Shea, D., Schachern, P., Paparella, M.M., & Carpenter, A.M. Passage of albumin from the middle ear to the inner ear in otitis media in the chinchilla. United States. doi:10.1016/S0196-0709(81)80017-8.
Goldberg, B., Goycoolea, M.V., Schleivert, P.M., Shea, D., Schachern, P., Paparella, M.M., and Carpenter, A.M. Sat . "Passage of albumin from the middle ear to the inner ear in otitis media in the chinchilla". United States. doi:10.1016/S0196-0709(81)80017-8.
@article{osti_5406850,
title = {Passage of albumin from the middle ear to the inner ear in otitis media in the chinchilla},
author = {Goldberg, B. and Goycoolea, M.V. and Schleivert, P.M. and Shea, D. and Schachern, P. and Paparella, M.M. and Carpenter, A.M.},
abstractNote = {A study of the permeability of the middle ear-inner ear interface for macromolecules was carried out in chinchillas with open and obstructed eustachian tubes utilizing tritiated human serum albumin and immunoelectrophoresis. Tritiated albumin was placed in the round window niche area or normal animals and animals in which the eustachian tubes had been obstructed for 24 hours or 14 days. The tritiated albumin was allowed to remain in the middle ear cavity for 24 hours, Samples of middle ear effusion, perilymph, blood and cerebrospinal fluid were collected and measured for radioactivity. Radioactivity was demonstrated in the perilymph. Samples of middle ear effusions and perilymph were also studied by immunoelectrophoresis with goat antihuman albumin. Albumin placed in the round window niche of an experimental animal could be recovered unchanged in the perilymph. The results suggest a pathophysiologic explanation for the association of otitis media and sensorineural hearing loss or endolymphatic hydrops.},
doi = {10.1016/S0196-0709(81)80017-8},
journal = {Am. J. Otolaryngol.; (United States)},
number = ,
volume = 2:3,
place = {United States},
year = {1981},
month = {8}
}