Fibroblast radiosensitivity versus acute and late normal skin responses in patients treated for breast cancer

Journal Article · Sun Jul 30 00:00:00 EDT 1995 · International Journal of Radiation Oncology, Biology and Physics

DOI:https://doi.org/10.1016/0360-3016(95)00068-A· OSTI ID:539411

Brock, W A; Wike, J; Tucker, S L ^[1]

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1. Univ. of Texas, Houston, TX (United States); and others

To determine if the radiosensitivity of normal human skin fibroblasts, measured in early passage cultures, is significantly correlated with the degree of acute or late normal skin damage in patients treated for breast cancer with radiotherapy. To test assay reproducibility, SF2 values derived from paired biopsies of the same patient (12 cases) were compared. A reasonably good correlation (p = 0.075) was obtained for SF2s determined by high dose-rate irradiations with immediated plating, but not for delayed plating or low dose-rate treatments. The median coefficient of variation in the replicate SF2s after high dose-rate treatment and immediate plating was 13%, suggesting that the poor correlation in paired SF2 values is due to the magnitude of the uncertainty in SF2 relative to the overall spread in SF2 values between patients (CV = 28%). Individual SF2 values and averaged values from patients with data from two biopsies were compared with the acute and late clinical reactions. A significant negative correlation was found between SF2 and relative clinical response, but only when averaged high dose-rate SF2 values and telangiectasia scores were compared. There was no significant correlation between average SF2 values and acute responses or between individual SF2 measurements and either the acute or late clinical response. The results of this study suggest that the degree of late telangiectasia is at least partially dependent upon the intrinsic cellular radiosensitivity of normal fibroblasts, but the relationship is not clear cut. Multiple replicate assays are necessary to obtain reliable estimates of fibroblast SF2 values using current techniques. 20 refs., 3 figs., 3 tabs.

• OSTI ID: 539411
• Journal Information: International Journal of Radiation Oncology, Biology and Physics, Vol. 32, Issue 5; Other Information: PBD: 30 Jul 1995
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
55 BIOLOGY AND MEDICINE
BASIC STUDIES
FIBROBLASTS
RADIOSENSITIVITY
BIOASSAY
RELIABILITY
FRACTIONATED IRRADIATION
LOW DOSE IRRADIATION
DOSE RATES
PATIENTS
TELANGIECTASIS
RADIOTHERAPY
SURVIVAL CURVES
GENETICS
MAMMARY GLANDS
NEOPLASMS
SKIN
RADIOSENSITIVITY EFFECTS
BIOLOGICAL RADIATION EFFECTS
DOSE-RESPONSE RELATIONSHIPS
RADIATION INJURIES
DELAYED RADIATION EFFECTS
BIOPSY
STATISTICS