Genetics of the complementary restriction systems DpnI and DpnII revealed by cloning and recombination in Streptococcus pneumoniae

Conference · Thu May 01 00:00:00 EDT 1986

OSTI ID:5393923

Lacks, S A; Mannarelli, B M; Springhorn, S S; Greenberg, B; de la Campa, A G

Transformation and cloning of the DpnI and DpnII endonuclease genes has clarified the genetic basis of the two restriction systems. Molecular cloning was carried out in the Gram-positive S. pneumoniae host/vector system. Cloned chromosomal fragments from both DpnI- and DpnII-producing strains were subjected to nucleotide sequence determination and were used as probes for DNA hybridization analysis. It was shown that the restriction enzyme phenotype of S. pneumoniae depended on an intercellular genetic cassette mechanism. In this review some aspects of the evolution of restriction systems in S. pneumoniae and other bacterial will be discussed. 42 refs., 7 figs., 1 tab.

• Research Organization: Brookhaven National Lab., Upton, NY (USA). Biology Dept.
• DOE Contract Number: AC02-76CH00016
• OSTI ID: 5393923
• Report Number(s): BNL-38437; CONF-8605175-1; ON: DE86014851
• Resource Relation: Conference: International conference on streptococcal genetics, Miami, FL, USA, 21 May 1986; Other Information: Portions of this document are illegible in microfiche products
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ENDONUCLEASES
DNA SEQUENCING
DNA-CLONING
BIOLOGICAL EVOLUTION
GEL PERMEATION CHROMATOGRAPHY
GENE REGULATION
GENETIC MAPPING
RECOMBINANT DNA
STREPTOCOCCUS
BACTERIA
CHROMATOGRAPHY
CLONING
DNA
DNA-ASE
ENZYMES
ESTERASES
HYDROLASES
MAPPING
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
SEPARATION PROCESSES
STRUCTURAL CHEMICAL ANALYSIS
550200* - Biochemistry
550700 - Microbiology