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Title: Detection of glycoproteins in the Acanthamoeba plasma membrane

Abstract

In the present study the authors have shown that glycoproteins are present in the plasma membrane of Acanthamoeba castellanii by utilizing different radioactive labeling techniques. Plasma membrane proteins in the amoeba were iodinated by {sup 125}I-lactoperoxidase labeling and the solubilized radiolabeled glycoproteins were separated by lectin-Sepharose affinity chromatography followed by polyacrylamide gel electrophoresis. The periodate/NaB{sup 3}H{sub 4} and galactose oxidase/NaB{sup 3}H{sub 4} labeling techniques were used for labeling of surface carbohydrates in the amoeba. Several surface-labeled glycoproteins were observed in addition to a diffusely labeled region with M{sub r} of 55,000-75,000 seen on electrophoresis, which could represent glycolipids. The presence of glycoproteins in the plasma membrane of Acanthamoeba castellanii was confirmed by metabolic labeling with ({sup 35}S)methionine followed by lectin-Sepharose affinity chromatography and polyacrylamide gel electrophoresis.

Authors:
 [1];  [2]
  1. (Abo Akademi (Finland))
  2. (Univ. of Helsinki (Finland))
Publication Date:
OSTI Identifier:
5393294
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research; (United States)
Additional Journal Information:
Journal Volume: 179:1; Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CELL MEMBRANES; MOLECULAR STRUCTURE; GLYCOPROTEINS; BIOCHEMISTRY; LABELLED COMPOUNDS; UPTAKE; AMOEBA; CARBOHYDRATES; ELECTROPHORESIS; IODINE 125; METHIONINE; PEROXIDASES; SULFUR 35; TRITIUM COMPOUNDS; AMINO ACIDS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBOXYLIC ACIDS; CELL CONSTITUENTS; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DRUGS; ELECTRON CAPTURE RADIOISOTOPES; ENZYMES; EVEN-ODD NUCLEI; HYDROGEN COMPOUNDS; INTERMEDIATE MASS NUCLEI; INTERNAL CONVERSION RADIOISOTOPES; INVERTEBRATES; IODINE ISOTOPES; ISOTOPES; LIGHT NUCLEI; LIPOTROPIC FACTORS; MEMBRANES; MICROORGANISMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; OXIDOREDUCTASES; PROTEINS; PROTOZOA; RADIOISOTOPES; SARCODINA; SULFUR ISOTOPES; 550301* - Cytology- Tracer Techniques

Citation Formats

Paatero, G.I.L., and Gahmberg, C.G. Detection of glycoproteins in the Acanthamoeba plasma membrane. United States: N. p., 1988. Web. doi:10.1016/0014-4827(88)90364-3.
Paatero, G.I.L., & Gahmberg, C.G. Detection of glycoproteins in the Acanthamoeba plasma membrane. United States. doi:10.1016/0014-4827(88)90364-3.
Paatero, G.I.L., and Gahmberg, C.G. Tue . "Detection of glycoproteins in the Acanthamoeba plasma membrane". United States. doi:10.1016/0014-4827(88)90364-3.
@article{osti_5393294,
title = {Detection of glycoproteins in the Acanthamoeba plasma membrane},
author = {Paatero, G.I.L. and Gahmberg, C.G.},
abstractNote = {In the present study the authors have shown that glycoproteins are present in the plasma membrane of Acanthamoeba castellanii by utilizing different radioactive labeling techniques. Plasma membrane proteins in the amoeba were iodinated by {sup 125}I-lactoperoxidase labeling and the solubilized radiolabeled glycoproteins were separated by lectin-Sepharose affinity chromatography followed by polyacrylamide gel electrophoresis. The periodate/NaB{sup 3}H{sub 4} and galactose oxidase/NaB{sup 3}H{sub 4} labeling techniques were used for labeling of surface carbohydrates in the amoeba. Several surface-labeled glycoproteins were observed in addition to a diffusely labeled region with M{sub r} of 55,000-75,000 seen on electrophoresis, which could represent glycolipids. The presence of glycoproteins in the plasma membrane of Acanthamoeba castellanii was confirmed by metabolic labeling with ({sup 35}S)methionine followed by lectin-Sepharose affinity chromatography and polyacrylamide gel electrophoresis.},
doi = {10.1016/0014-4827(88)90364-3},
journal = {Experimental Cell Research; (United States)},
issn = {0014-4827},
number = ,
volume = 179:1,
place = {United States},
year = {1988},
month = {11}
}