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Title: Effect of energy intake on the metabolism of glucose and glutamine in rumen epithelial tissue

Abstract

Ten Holstein steers (579 kg average body weight) were fed either alfalfa hay (12.2% crude protein) or a 90% concentrate diet to supply 14.2 or 25.2 Mcal ME respectively for a minimum of 28 days. Samples of rumen epithelial tissue were removed at slaughter from the anterior ventral sac, washed free of feed particles and transported to the laboratory in oxygenated Krebs-Ringer bicarbonate buffer (KRB; pH 7.4). Papillae were weighed (100-200 mg) in triplicate into flasks containing 3 ml KRB with 1 mM glutamine or 5 mM glucose and acetate (50 mM), propionate (25 mM), butyrate (15 mM), lactate (1 mM) and glucose (5 mM) or glutamine (1 mM) as competing substrates. A parallel set of flasks contained 1 or .5 ..mu..Ci of (U-/sup 14/C)-glutamine or glucose respectively for /sup 14/CO/sub 2/ production. There were no interactions with dietary energy intake and substrate addition. Increasing the dietary energy intake increased (P < .01) rates of uptake, /sup 14/CO/sub 2/ production and net lactate production from glucose and increased the /sup 14/CO/sub 2/ production from glutamine. Addition of acetate, propionate, butyrate and lactate decreased (P < .05) uptake of glucose, but only propionate decreased /sup 14/CO/sub 2/ production from glucose (40%).more » Addition of butyrate and glucose decreased /sup 14/CO/sub 2/ production from glutamine while propionate addition decreased net glutamate production and increased net alanine production. At these substrate concentrations rates of glucose oxidation to /sup 14/CO/sub 2/ were 7-fold higher than glutamine.« less

Authors:
Publication Date:
Research Org.:
Kansas State Univ., Manhattan
OSTI Identifier:
5392006
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA; TRN: 86-028436
Resource Type:
Conference
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GLUCOSE; METABOLISM; GLUTAMINE; ALANINES; BUTYRIC ACID; CARBON 14 COMPOUNDS; CARBON DIOXIDE; CATTLE; EPITHELIUM; LACTIC ACID; TRACER TECHNIQUES; ALDEHYDES; AMIDES; AMINO ACIDS; ANIMAL TISSUES; ANIMALS; BODY; CARBOHYDRATES; CARBON COMPOUNDS; CARBON OXIDES; CARBOXYLIC ACIDS; CHALCOGENIDES; DOMESTIC ANIMALS; HEXOSES; HYDROXY ACIDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; MONOCARBOXYLIC ACIDS; MONOSACCHARIDES; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; RUMINANTS; SACCHARIDES; TISSUES; VERTEBRATES; 550501* - Metabolism- Tracer Techniques

Citation Formats

Harmon, D L. Effect of energy intake on the metabolism of glucose and glutamine in rumen epithelial tissue. United States: N. p., 1986. Web.
Harmon, D L. Effect of energy intake on the metabolism of glucose and glutamine in rumen epithelial tissue. United States.
Harmon, D L. Sat . "Effect of energy intake on the metabolism of glucose and glutamine in rumen epithelial tissue". United States.
@article{osti_5392006,
title = {Effect of energy intake on the metabolism of glucose and glutamine in rumen epithelial tissue},
author = {Harmon, D L},
abstractNote = {Ten Holstein steers (579 kg average body weight) were fed either alfalfa hay (12.2% crude protein) or a 90% concentrate diet to supply 14.2 or 25.2 Mcal ME respectively for a minimum of 28 days. Samples of rumen epithelial tissue were removed at slaughter from the anterior ventral sac, washed free of feed particles and transported to the laboratory in oxygenated Krebs-Ringer bicarbonate buffer (KRB; pH 7.4). Papillae were weighed (100-200 mg) in triplicate into flasks containing 3 ml KRB with 1 mM glutamine or 5 mM glucose and acetate (50 mM), propionate (25 mM), butyrate (15 mM), lactate (1 mM) and glucose (5 mM) or glutamine (1 mM) as competing substrates. A parallel set of flasks contained 1 or .5 ..mu..Ci of (U-/sup 14/C)-glutamine or glucose respectively for /sup 14/CO/sub 2/ production. There were no interactions with dietary energy intake and substrate addition. Increasing the dietary energy intake increased (P < .01) rates of uptake, /sup 14/CO/sub 2/ production and net lactate production from glucose and increased the /sup 14/CO/sub 2/ production from glutamine. Addition of acetate, propionate, butyrate and lactate decreased (P < .05) uptake of glucose, but only propionate decreased /sup 14/CO/sub 2/ production from glucose (40%). Addition of butyrate and glucose decreased /sup 14/CO/sub 2/ production from glutamine while propionate addition decreased net glutamate production and increased net alanine production. At these substrate concentrations rates of glucose oxidation to /sup 14/CO/sub 2/ were 7-fold higher than glutamine.},
doi = {},
journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:3,
place = {United States},
year = {1986},
month = {3}
}

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