skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Glial expression of the {beta}-Amyloid Precursor Protein (APP) in global ischemia

Abstract

The {beta}-amyloid precursor protein (APP) bears characteristics of an acute-phase protein and therefore is likely to be involved in the glial response to brain injury. In the brain, APP is rapidly synthesized by activated glial cells in response to comparatively mild neuronal lesions, e.g., a remote peripheral nerve injury. Perfusion deficits in the brain result largely in neuronal necrosis and are a common condition in elderly patients. This neuronal necrosis is accompanied by a pronounced reaction of astrocytes and microglia, which can also be observed in animal models. We have therefore studied in the rat, immunocytochemically, the induction of APP after 30 min of global ischemia caused by four-vessel occlusion. The postischemic brain injuries were examined at survival times from 12 h to 7 days. From day 3 onward, APP immunoreactivity was strongly induced in the CA{sub 1} and CA{sub 4} regions of the rat dorsal hippocampus as well as in the dorsolateral striatum. In these areas, the majority of APP-immunoreactive cells were reactive glial fibrillary acidic protein (GFAP)-positive astrocytes, as shown by double-immunofluorescence labeling for GFAP and APP. Additionally, small ramified cells, most likely activated microglia, expressed APP immunoreactivity. In contrast, in the parietal cortex, APP immunoreactivity occurred focallymore » in clusters of activated microglia rather than in astrocytes, as demonstrated by double-immunofluorescence labeling for APP and the microglia-binding lectin Griffonia simplicifolia isolectin B{sub 4}. In conclusion, following global ischemia, APP is induced in reactive glial cells with spatial differences in the distribution pattern of APP induction in actrocytes and microglia. 51 refs., 4 figs.« less

Authors:
; ;  [1]
  1. Max Planck Institute of Psychiarty, Martinsried (Germany)
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
539147
Resource Type:
Journal Article
Journal Name:
Journal of Cerebral Blood Flow and Metabolism
Additional Journal Information:
Journal Volume: 15; Journal Issue: 4; Other Information: PBD: Jul 1995
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; PROTEINS; GENE REGULATION; ISCHEMIA; BIOLOGICAL MODELS; BRAIN; INJURIES; NECROSIS; NERVE CELLS; RATS; HIPPOCAMPUS; NERVOUS SYSTEM DISEASES; HEREDITARY DISEASES; MENTAL DISORDERS

Citation Formats

Banati, R B, Gehrmann, J, Kreutzberg, G W, Max Planck Institute for Neurological Research, Koeln, and Univ. Hospital, Zurich. Glial expression of the {beta}-Amyloid Precursor Protein (APP) in global ischemia. United States: N. p., 1995. Web. doi:10.1038/jcbfm.1995.80.
Banati, R B, Gehrmann, J, Kreutzberg, G W, Max Planck Institute for Neurological Research, Koeln, & Univ. Hospital, Zurich. Glial expression of the {beta}-Amyloid Precursor Protein (APP) in global ischemia. United States. doi:10.1038/jcbfm.1995.80.
Banati, R B, Gehrmann, J, Kreutzberg, G W, Max Planck Institute for Neurological Research, Koeln, and Univ. Hospital, Zurich. Sat . "Glial expression of the {beta}-Amyloid Precursor Protein (APP) in global ischemia". United States. doi:10.1038/jcbfm.1995.80.
@article{osti_539147,
title = {Glial expression of the {beta}-Amyloid Precursor Protein (APP) in global ischemia},
author = {Banati, R B and Gehrmann, J and Kreutzberg, G W and Max Planck Institute for Neurological Research, Koeln and Univ. Hospital, Zurich},
abstractNote = {The {beta}-amyloid precursor protein (APP) bears characteristics of an acute-phase protein and therefore is likely to be involved in the glial response to brain injury. In the brain, APP is rapidly synthesized by activated glial cells in response to comparatively mild neuronal lesions, e.g., a remote peripheral nerve injury. Perfusion deficits in the brain result largely in neuronal necrosis and are a common condition in elderly patients. This neuronal necrosis is accompanied by a pronounced reaction of astrocytes and microglia, which can also be observed in animal models. We have therefore studied in the rat, immunocytochemically, the induction of APP after 30 min of global ischemia caused by four-vessel occlusion. The postischemic brain injuries were examined at survival times from 12 h to 7 days. From day 3 onward, APP immunoreactivity was strongly induced in the CA{sub 1} and CA{sub 4} regions of the rat dorsal hippocampus as well as in the dorsolateral striatum. In these areas, the majority of APP-immunoreactive cells were reactive glial fibrillary acidic protein (GFAP)-positive astrocytes, as shown by double-immunofluorescence labeling for GFAP and APP. Additionally, small ramified cells, most likely activated microglia, expressed APP immunoreactivity. In contrast, in the parietal cortex, APP immunoreactivity occurred focally in clusters of activated microglia rather than in astrocytes, as demonstrated by double-immunofluorescence labeling for APP and the microglia-binding lectin Griffonia simplicifolia isolectin B{sub 4}. In conclusion, following global ischemia, APP is induced in reactive glial cells with spatial differences in the distribution pattern of APP induction in actrocytes and microglia. 51 refs., 4 figs.},
doi = {10.1038/jcbfm.1995.80},
journal = {Journal of Cerebral Blood Flow and Metabolism},
number = 4,
volume = 15,
place = {United States},
year = {1995},
month = {7}
}