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Title: Isolation and characterization of cryptic plasmids from filamentous, nonheterocystous cyanobacteria

Abstract

Many filamentous, nonheterocystous, N/sub 2/-fixing cyanobacteria from the LPP group contain cryptic plasmids. Plasmid number and size were determined as well as sequence homologies among independent strains of the same species, and different species. Only a few studied have been carried out to determine plasmid profiles in filamentous non-heterocystous cyanobacteria. Also, sequence homologies between plasmids have been examined only in the unicellular organisms. Using both liquid and solid BG-11 culture media, various strain specific, rapid, small-scale plasmid isolation procedures have been developed. These include lysozyme/SDS incubations, phenol and chloroform extractions, and an isopropanol precipitation. Resuspended pellets were electrophoresed on agarose gels, providing a quick and accurate method of determining plasmid size and number. Several endogenous plasmids have been isolated, cloned, and mapped through restriction enzyme digestion. These clones were used in Southern hybridizations to determine sequence homologies between plasmids of several strains and species.

Authors:
;
Publication Date:
Research Org.:
Miami Univ., Oxford, OH
OSTI Identifier:
5383688
Alternate Identifier(s):
OSTI ID: 5383688
Resource Type:
Conference
Journal Name:
Plant Physiol.; (United States)
Additional Journal Information:
Journal Volume: 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CYANOBACTERIA; HYBRIDIZATION; PLASMIDS; PROTEIN STRUCTURE; CLONING; ELECTROPHORESIS; VARIATIONS; CELL CONSTITUENTS; MICROORGANISMS 550700* -- Microbiology; 551000 -- Physiological Systems

Citation Formats

Felkner, R.H., and Barnum, S.R. Isolation and characterization of cryptic plasmids from filamentous, nonheterocystous cyanobacteria. United States: N. p., 1986. Web.
Felkner, R.H., & Barnum, S.R. Isolation and characterization of cryptic plasmids from filamentous, nonheterocystous cyanobacteria. United States.
Felkner, R.H., and Barnum, S.R. Tue . "Isolation and characterization of cryptic plasmids from filamentous, nonheterocystous cyanobacteria". United States.
@article{osti_5383688,
title = {Isolation and characterization of cryptic plasmids from filamentous, nonheterocystous cyanobacteria},
author = {Felkner, R.H. and Barnum, S.R.},
abstractNote = {Many filamentous, nonheterocystous, N/sub 2/-fixing cyanobacteria from the LPP group contain cryptic plasmids. Plasmid number and size were determined as well as sequence homologies among independent strains of the same species, and different species. Only a few studied have been carried out to determine plasmid profiles in filamentous non-heterocystous cyanobacteria. Also, sequence homologies between plasmids have been examined only in the unicellular organisms. Using both liquid and solid BG-11 culture media, various strain specific, rapid, small-scale plasmid isolation procedures have been developed. These include lysozyme/SDS incubations, phenol and chloroform extractions, and an isopropanol precipitation. Resuspended pellets were electrophoresed on agarose gels, providing a quick and accurate method of determining plasmid size and number. Several endogenous plasmids have been isolated, cloned, and mapped through restriction enzyme digestion. These clones were used in Southern hybridizations to determine sequence homologies between plasmids of several strains and species.},
doi = {},
journal = {Plant Physiol.; (United States)},
number = ,
volume = 80:4,
place = {United States},
year = {1986},
month = {4}
}

Conference:
Other availability
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