Identification of acetate groups on the backbone of rhamnogalacturonan I, a pectic polysaccharide of primary walls, obtained from suspension cultured cotton cells
Pectic polymers are an integral part of the primary cell walls of dicots. Pectins may play a role in cell expansion, cell adhesion, disease responses, and perhaps cell differentiation and recognition. The structure of pectins is complicated and is not fully characterized. A more detailed knowledge of their structure will allow a better understanding of the function of pectins. The disaccharide GalA-Rha which is the repeating unit of the backbone of the polymer was isolated by treating the cell walls with anhydrous liquid HF at -23 C for 30 minutes. Liquid HF under these conditions extracts all of the sugar from the walls except those in cellulose and the homogalacturonan region of pectins while retaining the acyl substituents on the sugars. This treatment takes advantage of the enormous difference in lability in HF of rhamnosyl and galacturonosyl linkages. /sup 1/H NMR spectra of the disaccharide show that around one in 2 of the repeat units is acetylated. Preliminary results from methylation analysis indicate that the acetate group is most likely on the 2 or 3 position of the galacturonic acid.
- Research Organization:
- Oklahoma State Univ., Stillwater
- OSTI ID:
- 5383261
- Journal Information:
- Plant Physiol.; (United States), Vol. 80:4; Conference: Annual meeting of the American Society of Plant Physiologists, Baton Rouge, LA, USA, 8-12 Jun 1986
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
PECTINS
BIOLOGICAL FUNCTIONS
NUCLEAR MAGNETIC RESONANCE
STRUCTURAL CHEMICAL ANALYSIS
CELL CULTURES
COTTON PLANTS
DEUTERIUM
BLOOD SUBSTITUTES
CARBOHYDRATES
DRUGS
FUNCTIONS
HEMATOLOGIC AGENTS
HYDROGEN ISOTOPES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
RESONANCE
SACCHARIDES
STABLE ISOTOPES
551000* - Physiological Systems
550600 - Medicine