Nutritional complementation of oxidative glucose metabolism in Escherichia coli via pyrroloquinoline quinone-dependent glucose dehydrogenase and the Entner-Doudoroff pathway

Journal Article · Mon Jul 01 00:00:00 EDT 1991 · Applied and Environmental Microbiology; (United States)

OSTI ID:5382625

Adamowicz, M; Conway, T; Nickerson, K W ^[1]

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1. Univ. of Nebraska, Lincoln (USA)

Two glucose-negative Escherichia coli mutants (ZSC113 and DF214) were unable to grow on glucose as the sole carbon source unless supplemented with pyrroloquinoline quinone (PQQ). PQQ is the cofactor for the periplasmic enzyme glucose dehydrogenase, which converts glucose to gluconate. Aerobically, E. Coli ZSC113 grew on glucose plus PQQ with a generation time of 65 min, a generation time about the same as that for wild-type E. coli in a defined glucose-salts medium. Thus, for E. coli ZSC113 the Entner-Doudoroff pathway was fully able to replace the Embden-Meyerhof-Parnas pathway. In the presence of 5% sodium dodecyl sulfate, PQQ no longer acted as a growth factor. Sodium dodecyl sulfate inhibited the formation of gluconate from glucose but not gluconate metabolism. Adaptation to PQQ-dependent growth exhibited long lag periods, except under low-phosphate conditions, in which the PhoE porin would be expressed. The authors suggest that E. coli has maintained the apoenzyme for glucose dehydrogenase and the Entner-Doudoroff pathway as adaptations to an aerobic, low-phosphate, and low-detergent aquatic environment.

• DOE Contract Number: FG02-90ER20002
• OSTI ID: 5382625
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 57:7; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
ESCHERICHIA COLI
BIOLOGICAL PATHWAYS
AEROBIC CONDITIONS
BIOLOGICAL ADAPTATION
COENZYMES
ENVIRONMENT
GLUCOSE
METABOLISM
OXIDOREDUCTASES
ALDEHYDES
BACTERIA
CARBOHYDRATES
ENZYMES
HEXOSES
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
SACCHARIDES
540310* - Environment
Aquatic- Basic Studies- (1990-)