Exposure of PC12 cells to NGF/ethanol results in accelerated differentiation and altered gene expression
- Auburn Univ., AL (United States)
The role of alcohols in affecting neuromorphogenesis was investigated in the pheochromocytoma cell line, PC12. The effect of ethanol at physiological concentrations in this system leads to accelerated neurite extension in the presence of suboptimal concentrations of NGF. Accelerated morphological differentiation was dependent upon the side chain length of the alcohol and not inhibited by pyrazole. Ethanol/NGF induced neurite extension can be blocked with 50nM of K252a, but not sphingozine, H7, H89, genistein or okadaic acid. Changes in the expression of 17 NGF-induced and/or neuronal transcripts were examined in relationship to time of NGF/ethanol exposure; dose of NGF/ethanol; and side-chain length of NFG/alcohol. The authors studies indicate that ethanol potentiates the effects of NFG and subsequent neurogenesis through both protein kinase C and cAMP-independent pathways. In addition, these data show that ethanol is capable of altering gene expression in a specific manner.
- OSTI ID:
- 5374490
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ETHANOL
BIOLOGICAL EFFECTS
GROWTH FACTORS
BIOLOGICAL FUNCTIONS
TUMOR CELLS
CELL DIFFERENTIATION
BIOLOGICAL PATHWAYS
GENE REGULATION
MOLECULAR STRUCTURE
PHOSPHOTRANSFERASES
ALCOHOLS
ANIMAL CELLS
ENZYMES
HYDROXY COMPOUNDS
MITOGENS
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
TRANSFERASES
560300* - Chemicals Metabolism & Toxicology