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Title: Rapid and sensitive radioassay for dihydroorotase

Abstract

A rapid and simple radiochemical assay for dihydroorotase (DHOase; EC 3.5.2.3) has been developed. DHOase activity is assessed by measuring the amount of L-5,6-dihydro(/sup 14/C)orotate formed from N(/sup 14/C)cerbamyl-L-aspartate in the forward reaction and N(/sup 14/C)carbamly-L-aspartate formed from L-5,6-dihydro(/sup 14/C)orotate in the reverse reaction following the separation of the two compounds by high-voltage paper electrophoresis in an acetic acid/formic acid solution, pH 2.30. The procedure can, in principle, also be applied to the assay of aspartate carbamyl transferase (EC 2.1.3.2) and dihydroorotate dehydrogenase (EC 1.3.3.1).

Authors:
;
Publication Date:
Research Org.:
Univ. of New South Wales, Kinsington
OSTI Identifier:
5367780
Resource Type:
Journal Article
Journal Name:
J. Appl. Biochem.; (United States)
Additional Journal Information:
Journal Volume: 1:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ENZYMES; RADIOASSAY; CARBON 14; DEHYDROGENASES; TRANSFERASES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARBON ISOTOPES; EVEN-EVEN NUCLEI; ISOTOPES; LIGHT NUCLEI; NUCLEI; OXIDOREDUCTASES; RADIOISOTOPES; YEARS LIVING RADIOISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Finney, K.G., and O'Sullivan, W.J. Rapid and sensitive radioassay for dihydroorotase. United States: N. p., 1979. Web.
Finney, K.G., & O'Sullivan, W.J. Rapid and sensitive radioassay for dihydroorotase. United States.
Finney, K.G., and O'Sullivan, W.J. Thu . "Rapid and sensitive radioassay for dihydroorotase". United States.
@article{osti_5367780,
title = {Rapid and sensitive radioassay for dihydroorotase},
author = {Finney, K.G. and O'Sullivan, W.J.},
abstractNote = {A rapid and simple radiochemical assay for dihydroorotase (DHOase; EC 3.5.2.3) has been developed. DHOase activity is assessed by measuring the amount of L-5,6-dihydro(/sup 14/C)orotate formed from N(/sup 14/C)cerbamyl-L-aspartate in the forward reaction and N(/sup 14/C)carbamly-L-aspartate formed from L-5,6-dihydro(/sup 14/C)orotate in the reverse reaction following the separation of the two compounds by high-voltage paper electrophoresis in an acetic acid/formic acid solution, pH 2.30. The procedure can, in principle, also be applied to the assay of aspartate carbamyl transferase (EC 2.1.3.2) and dihydroorotate dehydrogenase (EC 1.3.3.1).},
doi = {},
journal = {J. Appl. Biochem.; (United States)},
number = ,
volume = 1:1,
place = {United States},
year = {1979},
month = {2}
}