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Title: Concurrent birefringence and forward light-scattering measurements of flash-bleached rod outer segments

Abstract

A microretardometer--nephelometer was constructed to measure birefringence and forward-direction light scattering concurrently in single retinal rod outer segments (ROS). The relative contributions of light-induced birefringence and light-scattering changes to observed far-red transmission changes measured normal to the cell axis were evaluated in isolated, whole, flash-bleached ROS. No light-scattering transients were found corresponding to the previously reported rapid birefringence loss associated with the metarhodopsin I lead to metarhodopsin II reaction even under conditions in which such a light-scattering change has been reported for outer-segment fragments.

Authors:
Publication Date:
Research Org.:
Neurological Sciences Institute, Good Samaritan Hospital and Medical Center, Portland, Oregon
OSTI Identifier:
5364542
Resource Type:
Journal Article
Journal Name:
J. Opt. Soc. Am.; (United States)
Additional Journal Information:
Journal Volume: 71:12
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; RETINA; OPTICAL PROPERTIES; BIOCHEMICAL REACTION KINETICS; BIREFRINGENCE; LIGHT SCATTERING; OPHTHALMOLOGY; PHYSIOLOGY; VISION; BODY; BODY AREAS; EYES; FACE; HEAD; KINETICS; MEDICINE; ORGANS; PHYSICAL PROPERTIES; REACTION KINETICS; REFRACTION; SCATTERING; SENSE ORGANS; 551000* - Physiological Systems; 550200 - Biochemistry

Citation Formats

Kaplan, M W. Concurrent birefringence and forward light-scattering measurements of flash-bleached rod outer segments. United States: N. p., 1981. Web. doi:10.1364/JOSA.71.001467.
Kaplan, M W. Concurrent birefringence and forward light-scattering measurements of flash-bleached rod outer segments. United States. doi:10.1364/JOSA.71.001467.
Kaplan, M W. Tue . "Concurrent birefringence and forward light-scattering measurements of flash-bleached rod outer segments". United States. doi:10.1364/JOSA.71.001467.
@article{osti_5364542,
title = {Concurrent birefringence and forward light-scattering measurements of flash-bleached rod outer segments},
author = {Kaplan, M W},
abstractNote = {A microretardometer--nephelometer was constructed to measure birefringence and forward-direction light scattering concurrently in single retinal rod outer segments (ROS). The relative contributions of light-induced birefringence and light-scattering changes to observed far-red transmission changes measured normal to the cell axis were evaluated in isolated, whole, flash-bleached ROS. No light-scattering transients were found corresponding to the previously reported rapid birefringence loss associated with the metarhodopsin I lead to metarhodopsin II reaction even under conditions in which such a light-scattering change has been reported for outer-segment fragments.},
doi = {10.1364/JOSA.71.001467},
journal = {J. Opt. Soc. Am.; (United States)},
number = ,
volume = 71:12,
place = {United States},
year = {1981},
month = {12}
}