Excision repair of UV radiation-induced DNA damage in Caenorhabditis elegans

Journal Article · Thu Jun 01 00:00:00 EDT 1989 · Genetics; (USA)

OSTI ID:5363931

Hartman, P S; Hevelone, J; Dwarakanath, V; Mitchell, D L ^[1]

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1. Texas Christian Univ., Fort Worth (USA)

Radioimmunoassays were used to monitor the removal of antibody-binding sites associated with the two major UV radiation-induced DNA photoproducts (cyclobutane dimers and (6-4) photoproducts). Unlike with cultured human cells, where (6-4) photoproducts are removed more rapidly than cyclobutane dimers, the kinetics of repair were similar for both lesions. Repair capacity in wild type diminished throughout development. The radioimmunoassays were also employed to confirm the absence of photoreactivation in C. elegans. In addition, three radiation-sensitive mutants (rad-1, rad-2, rad-7) displayed normal repair capacities. An excision defect was much more pronounced in larvae than embryos in the fourth mutant tested (rad-3). This correlates with the hypersensitivity pattern of this mutant and suggests that DNA repair may be developmentally regulated in C. elegans. The mechanism of DNA repair in C. elegans as well as the relationship between the repair of specific photoproducts and UV radiation sensitivity during development are discussed.

• OSTI ID: 5363931
• Journal Information: Genetics; (USA), Vol. 122:2; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
NEMATODES
EXCISION REPAIR
RECEPTORS
RADIOIMMUNOASSAY
BIOCHEMICAL REACTION KINETICS
DNA
LARVAE
PHOTOCHEMISTRY
PYRIMIDINE DIMERS
RADIATION INDUCED MUTANTS
ULTRAVIOLET RADIATION
ASCHELMINTHES
BIOASSAY
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CHEMISTRY
DIAGNOSTIC TECHNIQUES
DNA REPAIR
ELECTROMAGNETIC RADIATION
HELMINTHS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KINETICS
MEMBRANE PROTEINS
MUTANTS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PROTEINS
RADIATIONS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
REACTION KINETICS
RECOVERY
REPAIR
TRACER TECHNIQUES
550201* - Biochemistry- Tracer Techniques