Quantitation of postischemic skeletal muscle necrosis: histochemical and radioisotope techniques
Abstract
Skeletal muscle necrosis will result from prolonged periods of ischemia. The purpose of this study was to develop a method to estimate the extent of necrosis using nitroblue tetrazolium staining and technetium scanning. The bilateral canine gracilis muscle preparation with total vascular isolation was exposed to 4 hr of complete normothermic ischemia followed by reperfusion. After 45 hr of reperfusion /sup 99m/Tc pyrophosphate (PYP) was injected and 3 hr later the muscles were harvested, cut into six slices, and stained with nitroblue tetrazolium. Biopsies were taken from tetrazolium-positive and -negative areas for electron microscopy to confirm the ability of the stain to distinguish viable from necrotic muscle. Computerized planimetry of the staining pattern was used to estimate the extent of necrosis as a percentage of the total muscle. Electron microscopy confirmed the validity of nitroblue tetrazolium to discriminate between viable and necrotic skeletal muscle in this experimental model. After 4 hr of ischemia the percentage necrosis was 30.2 +/- 6.1% (mean +/- SEM, n = 12), there was no difference in the extent of necrosis in left vs right paired muscles, using tetrazolium staining or technetium PYP uptake. There was a statistically significant correlation between the percentage necrosis and themore »
- Authors:
- Publication Date:
- Research Org.:
- Toronto General Hospital, Ontario
- OSTI Identifier:
- 5362358
- Resource Type:
- Journal Article
- Journal Name:
- J. Surg. Res.; (United States)
- Additional Journal Information:
- Journal Volume: 44:1
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 62 RADIOLOGY AND NUCLEAR MEDICINE; ISCHEMIA; PATHOGENESIS; MUSCLES; NECROSIS; NUCLEAR MEDICINE; TRACER TECHNIQUES; SKELETAL DISEASES; DIAGNOSIS; BIOLOGICAL MODELS; DOGS; ELECTRON MICROSCOPY; IN VITRO; ISOMERIC NUCLEI; MEASURING METHODS; PYROPHOSPHATES; RADIOISOTOPE SCANNING; STAINS; TECHNETIUM 99; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; CARDIOVASCULAR DISEASES; COUNTING TECHNIQUES; DISEASES; HOURS LIVING RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; ISOMERIC TRANSITION ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MEDICINE; MICROSCOPY; NUCLEI; ODD-EVEN NUCLEI; OXYGEN COMPOUNDS; PATHOLOGICAL CHANGES; PHOSPHORUS COMPOUNDS; RADIOISOTOPES; TECHNETIUM ISOTOPES; VASCULAR DISEASES; VERTEBRATES; YEARS LIVING RADIOISOTOPES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics
Citation Formats
Labbe, R, Lindsay, T, Gatley, R, Romaschin, A, Mickle, D, Wilson, G, Houle, S, and Walker, P. Quantitation of postischemic skeletal muscle necrosis: histochemical and radioisotope techniques. United States: N. p., 1988.
Web. doi:10.1016/0022-4804(88)90121-7.
Labbe, R, Lindsay, T, Gatley, R, Romaschin, A, Mickle, D, Wilson, G, Houle, S, & Walker, P. Quantitation of postischemic skeletal muscle necrosis: histochemical and radioisotope techniques. United States. https://doi.org/10.1016/0022-4804(88)90121-7
Labbe, R, Lindsay, T, Gatley, R, Romaschin, A, Mickle, D, Wilson, G, Houle, S, and Walker, P. 1988.
"Quantitation of postischemic skeletal muscle necrosis: histochemical and radioisotope techniques". United States. https://doi.org/10.1016/0022-4804(88)90121-7.
@article{osti_5362358,
title = {Quantitation of postischemic skeletal muscle necrosis: histochemical and radioisotope techniques},
author = {Labbe, R and Lindsay, T and Gatley, R and Romaschin, A and Mickle, D and Wilson, G and Houle, S and Walker, P},
abstractNote = {Skeletal muscle necrosis will result from prolonged periods of ischemia. The purpose of this study was to develop a method to estimate the extent of necrosis using nitroblue tetrazolium staining and technetium scanning. The bilateral canine gracilis muscle preparation with total vascular isolation was exposed to 4 hr of complete normothermic ischemia followed by reperfusion. After 45 hr of reperfusion /sup 99m/Tc pyrophosphate (PYP) was injected and 3 hr later the muscles were harvested, cut into six slices, and stained with nitroblue tetrazolium. Biopsies were taken from tetrazolium-positive and -negative areas for electron microscopy to confirm the ability of the stain to distinguish viable from necrotic muscle. Computerized planimetry of the staining pattern was used to estimate the extent of necrosis as a percentage of the total muscle. Electron microscopy confirmed the validity of nitroblue tetrazolium to discriminate between viable and necrotic skeletal muscle in this experimental model. After 4 hr of ischemia the percentage necrosis was 30.2 +/- 6.1% (mean +/- SEM, n = 12), there was no difference in the extent of necrosis in left vs right paired muscles, using tetrazolium staining or technetium PYP uptake. There was a statistically significant correlation between the percentage necrosis and the density of 99mTc PYP uptake per muscle (r = 0.83, P less than 0.001) and per slice (r = 0.94, P less than 0.001). This study demonstrates the ability of tetrazolium staining to accurately differentiate between viable and necrotic skeletal muscle and provides a reproducible method for estimating the extent of necrosis in the gracilis muscle model.},
doi = {10.1016/0022-4804(88)90121-7},
url = {https://www.osti.gov/biblio/5362358},
journal = {J. Surg. Res.; (United States)},
number = ,
volume = 44:1,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1988},
month = {Fri Jan 01 00:00:00 EST 1988}
}