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Title: In vitro phagocytosis and intracellular survival of Campylobacter jejuni with phagocytes

Abstract

In vitro phagocytosis and intracellular survival of Campylobacter jejuni was studied using three types of mononuclear phagocytes: a J774G8 peritoneal macrophage line, resident BABL/c peritoneal macrophages and human peripheral blood monocytes. In phagocytosis assays using CFU determinations, phagocytosis increased steadily over an 8 hr time period. Results obtained using a /sup 51/Cr assay indicated no consistent significant difference between phagocytosis of C. jejuni between the three mononuclear phagocytes or PMN's and that maximum infection occurred prior to 0.5 hr and maintained throughout the 4 hr assay. Further investigation of the mechanism of attachment and entry of C. jejuni revealed this process required the expenditure of energy by the phagocyte, but was not inhibited by inhibitors of microfilament functions. In addition, phagocytosis was enhanced by the presence of 20% FCS,

Authors:
Publication Date:
Research Org.:
Chicago Medical School, IL (USA)
OSTI Identifier:
5348620
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BACTERIA; PHAGOCYTOSIS; PHAGOCYTES; BIOLOGICAL FUNCTIONS; CHROMIUM 51; IN VITRO; MACROPHAGES; MONOCYTES; NEUTROPHILS; SURVIVAL CURVES; TRACER TECHNIQUES; ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CHROMIUM ISOTOPES; CONNECTIVE TISSUE CELLS; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; FUNCTIONS; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; MATERIALS; MICROORGANISMS; NUCLEI; RADIOISOTOPES; SOMATIC CELLS; 550301* - Cytology- Tracer Techniques

Citation Formats

Kiehlbauch, J A. In vitro phagocytosis and intracellular survival of Campylobacter jejuni with phagocytes. United States: N. p., 1986. Web.
Kiehlbauch, J A. In vitro phagocytosis and intracellular survival of Campylobacter jejuni with phagocytes. United States.
Kiehlbauch, J A. Wed . "In vitro phagocytosis and intracellular survival of Campylobacter jejuni with phagocytes". United States.
@article{osti_5348620,
title = {In vitro phagocytosis and intracellular survival of Campylobacter jejuni with phagocytes},
author = {Kiehlbauch, J A},
abstractNote = {In vitro phagocytosis and intracellular survival of Campylobacter jejuni was studied using three types of mononuclear phagocytes: a J774G8 peritoneal macrophage line, resident BABL/c peritoneal macrophages and human peripheral blood monocytes. In phagocytosis assays using CFU determinations, phagocytosis increased steadily over an 8 hr time period. Results obtained using a /sup 51/Cr assay indicated no consistent significant difference between phagocytosis of C. jejuni between the three mononuclear phagocytes or PMN's and that maximum infection occurred prior to 0.5 hr and maintained throughout the 4 hr assay. Further investigation of the mechanism of attachment and entry of C. jejuni revealed this process required the expenditure of energy by the phagocyte, but was not inhibited by inhibitors of microfilament functions. In addition, phagocytosis was enhanced by the presence of 20% FCS,},
doi = {},
url = {https://www.osti.gov/biblio/5348620}, journal = {},
number = ,
volume = ,
place = {United States},
year = {1986},
month = {1}
}

Thesis/Dissertation:
Other availability
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