In vitro expansion of the murine pluripotent hemopoietic stem cell population in response to interleukin 3 and interleukin 6. Application to bone marrow transplantation

Journal Article · Fri Sep 01 00:00:00 EDT 1989 · Transplantation; (USA)

DOI:https://doi.org/10.1097/00007890-198909000-00028· OSTI ID:5347311

Okano, A; Suzuki, C; Takatsuki, F; Akiyama, Y; Koike, K; Ozawa, K; Hirano, T; Kishimoto, T; Nakahata, T; Asano, S ^[1]

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1. Ajinomoto Co., Inc., Kawasaki (Japan)

The synergistic action of interleukin 6 with interleukin 3 on the proliferation of a murine hemopoietic stem cell population in a short-term liquid culture system was examined by radioprotective assay. The numbers of colony-forming units in spleen (CFU-S), together with granulocyte/macrophage colony-forming units and viable nucleated cells, were found to increase markedly in culture in the presence of both IL-3 and IL-6, compared with the presence of IL-3 or IL-6 alone. The peak CFU-S value in response to the combination of IL-3 and IL-6 was obtained 6 days after culture initiation, exceeding 5-fold of the input value. Consistent with these data, marrow cells cultured with both IL-3 and IL-6 for 6 days were shown to have a much higher capability of rescuing lethally irradiated mice than did controls. The results may portend the potential clinical use of the combination of IL-3 and IL-6, in particular, in bone marrow transplantation.

• OSTI ID: 5347311
• Journal Information: Transplantation; (USA), Vol. 48:3; ISSN 0041-1337
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BONE MARROW CELLS
TRANSPLANTS
LYMPHOKINES
RADIOSENSITIVITY EFFECTS
STEM CELLS
COLONY FORMATION
CELL DIVISION
HEMATOPOIETIC SYSTEM
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ANIMAL CELLS
ANIMALS
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CONNECTIVE TISSUE CELLS
GROWTH FACTORS
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ORGANIC COMPOUNDS
PROTEINS
RODENTS
SOMATIC CELLS
VERTEBRATES
560152* - Radiation Effects on Animals- Animals