Radiation-induced micronuclei in peripheral erythrocytes of Rana catesbeiana: an aquatic animal model for in vivo genotoxicity studies
An in vivo micronucleus assay for peripheral erythrocytes of Rana catesbeiana tadpoles was developed and evaluated. The assay was used to determine the spontaneous frequency of micronuclei in circulating erythrocytes in tadpoles from two different populations, to define the time from administering the clastogen to the maximum micronucleus frequency in peripheral erythrocytes, and to determine the response to radiation. The spontaneous frequency of micronuclei in circulating erythrocytes of early-stage tadpoles was low, but higher than that of late-stage tadpoles. The time from the exposure of early-stage tadpoles to radiation (2.1 Gy) to the maximum micronucleus frequency was about 2 wk. The increase in frequency of micronuclei in peripheral erythrocytes of late-stage tadpoles receiving doses ranging from 0.5 to 3.0 Gy was linear with dose; a 3-fold increase was obtained with a dose of 3.0 Gy. The spontaneous frequency of micronuclei in erythrocytes and the increase in frequency induced by radiation appeared to differ in tadpoles from different populations. Quantification of micronuclei in the peripheral erythrocytes of R castesbeiana tadpoles provides a promising whole-animal system for studies of genotoxicity in aquatic environments.
- Research Organization:
- Lawrence Livermore National Lab., CA
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 5341636
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 10:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CHROMOSOMAL ABERRATIONS
RADIOINDUCTION
ERYTHROCYTES
BIOLOGICAL RADIATION EFFECTS
MUTAGENESIS
BIOLOGICAL MODELS
AGE DEPENDENCE
AQUATIC ORGANISMS
CELL NUCLEI
FROGS
GENETIC EFFECTS
IN VIVO
IONIZING RADIATIONS
LARVAE
AMPHIBIANS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
MATERIALS
MUTATIONS
RADIATION EFFECTS
RADIATIONS
VERTEBRATES
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture