Characteristics of the norepinephrine-stimulated phosphatidylinositol turnover in rat pineal cell dispersions

Journal Article · Thu Oct 01 00:00:00 EDT 1981 · Neurochem. Res.; (United States)

DOI:https://doi.org/10.1007/BF00964413· OSTI ID:5335676

Hauser, G; Smith, T L

Dispersed rat pineal cells can be used for the study of the phosphatidylinositol effect. The response to ( - )-norepinephrine of the incorporation of 32Pi into phospholipids is linear with time and cell concentration, stereospecific, and mediated through alpha-1-adrenergic receptors. Na+ in the incubation medium is obligatory for labeling of phosphatidylinositol and phosphatidylcholine by 32P. In the absence of K+, incorporation of 32P is drastically lowered and no stimulation by norepinephrine occurs. Rb+ can replace K+. Omission of Ca2+ or substitution with Sr2+ preferentially lowers incorporation of radioactivity into phosphatidylcholine. Mg2+ is not required for basal or stimulated labeling.

• Research Organization: Ralph Lowell Laboratories, McLean Hospital, Belmont, Massachusetts
• OSTI ID: 5335676
• Journal Information: Neurochem. Res.; (United States), Vol. 6:10
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
INOSITOLS
CHEMICAL ACTIVATION
NORADRENALINE
PHYSIOLOGY
PHOSPHOLIPIDS
ANIMAL CELLS
CALCIUM COMPOUNDS
CATIONS
MAGNESIUM COMPOUNDS
PINEAL GLAND
POTASSIUM COMPOUNDS
RECEPTORS
SODIUM COMPOUNDS
ADRENAL HORMONES
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CARBOHYDRATES
CARDIOTONICS
CARDIOVASCULAR AGENTS
CHARGED PARTICLES
DRUGS
ESTERS
GLANDS
HORMONES
IONS
LIPIDS
MONOSACCHARIDES
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
SACCHARIDES
STEROID HORMONES
SYMPATHOMIMETICS
551000* - Physiological Systems
550200 - Biochemistry