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Title: Nuclear lamins and peripheral nuclear antigens during fertilization and embryogenesis in mice and sea urchins

Abstract

Nuclear structural changes during fertilization and embryogenesis in mice and sea urchins are traced using four antibodies. The oocytes from virgin female mice, morulae and blastocytes from mated females, and gametes from the sea urchin Lytechnius variegatis are studied using mouse monoclonal antibodies to nuclear lamin A/C, monoclonal antibody to P1, human autoimmune antibodies to lamin A/C, and to lamin B. The mouse fertilization data reveal no lamins on the oocyte; however, lamins are present on the pronuclei, and chromosomes are found on the oocytes and pronuclei. It is detected that on the sea urchin sperm the lamins are reduced to acrosomal and centriolar fossae and peripheral antigens are around the sperm nucleus. The mouse sperm bind lamin antibodies regionally and do not contain antigens. Lamins and antigens are observed on both pronuclei and chromosomes during sea urchin fertilization. Mouse embryogenesis reveals that lamin A/C is not recognized at morula and blastocyst stages; however, lamin B stains are retained. In sea urchin embryogenesis lamin recognition is lost at the blastrula, gastrula, and plutei stages. It is noted that nuclear lamins lost during spermatogenesis are restored at fertilization and peripheral antigens are associated with the surface of chromosomes during meiosis andmore » mitosis and with the periphery of the pronuclei and nuclei during interphase. 32 references.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Florida State Univ., Tallahassee; Wistar Institute of Anatomy and Biology, Philadelphia, PA; Ottawa Univ., Canada
OSTI Identifier:
5327849
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 82
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; SEA URCHINS; FERTILIZATION; ANTIGENS; CHROMOSOMES; CYTOLOGY; MICE; MORPHOLOGY; NUCLEI; PROTEINS; ANIMALS; AQUATIC ORGANISMS; BIOLOGY; ECHINODERMS; INVERTEBRATES; MAMMALS; ORGANIC COMPOUNDS; RODENTS; VERTEBRATES; 551000* - Physiological Systems

Citation Formats

Schatten, G., Schatten, H., Simerly, C., Maul, G.G., and Chaly, N. Nuclear lamins and peripheral nuclear antigens during fertilization and embryogenesis in mice and sea urchins. United States: N. p., 1985. Web. doi:10.1073/pnas.82.14.4727.
Schatten, G., Schatten, H., Simerly, C., Maul, G.G., & Chaly, N. Nuclear lamins and peripheral nuclear antigens during fertilization and embryogenesis in mice and sea urchins. United States. doi:10.1073/pnas.82.14.4727.
Schatten, G., Schatten, H., Simerly, C., Maul, G.G., and Chaly, N. Mon . "Nuclear lamins and peripheral nuclear antigens during fertilization and embryogenesis in mice and sea urchins". United States. doi:10.1073/pnas.82.14.4727.
@article{osti_5327849,
title = {Nuclear lamins and peripheral nuclear antigens during fertilization and embryogenesis in mice and sea urchins},
author = {Schatten, G. and Schatten, H. and Simerly, C. and Maul, G.G. and Chaly, N.},
abstractNote = {Nuclear structural changes during fertilization and embryogenesis in mice and sea urchins are traced using four antibodies. The oocytes from virgin female mice, morulae and blastocytes from mated females, and gametes from the sea urchin Lytechnius variegatis are studied using mouse monoclonal antibodies to nuclear lamin A/C, monoclonal antibody to P1, human autoimmune antibodies to lamin A/C, and to lamin B. The mouse fertilization data reveal no lamins on the oocyte; however, lamins are present on the pronuclei, and chromosomes are found on the oocytes and pronuclei. It is detected that on the sea urchin sperm the lamins are reduced to acrosomal and centriolar fossae and peripheral antigens are around the sperm nucleus. The mouse sperm bind lamin antibodies regionally and do not contain antigens. Lamins and antigens are observed on both pronuclei and chromosomes during sea urchin fertilization. Mouse embryogenesis reveals that lamin A/C is not recognized at morula and blastocyst stages; however, lamin B stains are retained. In sea urchin embryogenesis lamin recognition is lost at the blastrula, gastrula, and plutei stages. It is noted that nuclear lamins lost during spermatogenesis are restored at fertilization and peripheral antigens are associated with the surface of chromosomes during meiosis and mitosis and with the periphery of the pronuclei and nuclei during interphase. 32 references.},
doi = {10.1073/pnas.82.14.4727},
journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 82,
place = {United States},
year = {1985},
month = {7}
}