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Title: Phorbol esters alter adenylate cyclase responses to vasoactive intestinal peptide and forskolin in the GH cell line

Abstract

Activation of protein kinase C with phorbol ester modifies cyclic AMP production in several anterior pituitary cell systems. In the GH cell line from a rat pituitary tumor, exposure to phorbol 12-myristate 13-acetate (PMA: 100 nM) for 30 minutes significantly reduces vasoactive intestinal peptide (VIP: 100 nM) stimulated adenylate cyclase (AC) activity in subsequent membrane preparations to 62 + 4% of control (n = 6 independent studies). In contrast, these same membrane preparations respond to forskolin (1 ..mu..M) with significantly more activity, 130 +/- 6% of controls (n = 6 independent studies). Finally, phorbol ester does not block an inhibitory hormone input into the AC system; somatostatin (100 nM) reduction of VIP-stimulated AC activity is not significantly different in membrane preparations from PMA treated and control cells (n = 3 independent studies). These other findings lead the authors to propose that protein kinase C can modify several sites in the AC complex in anterior pituitary cells.

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Virginia, Charlottesville
OSTI Identifier:
5325575
Alternate Identifier(s):
OSTI ID: 5325575
Report Number(s):
CONF-8606151-
Journal ID: CODEN: FEPRA
Resource Type:
Conference
Journal Name:
Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
Additional Journal Information:
Journal Volume: 45:6; Conference: 76. annual meeting of the Federation of American Society for Experimental Biology, Washington, DC, USA, 8 Jun 1986
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CYCLASES; ENZYME ACTIVITY; PHORBOL ESTERS; BIOLOGICAL EFFECTS; AMP; NEOPLASMS; PEPTIDES; PHOSPHOTRANSFERASES; PITUITARY GLAND; RATS; ANIMALS; BODY; CARCINOGENS; DISEASES; ENDOCRINE GLANDS; ENZYMES; ESTERS; GLANDS; LYASES; MAMMALS; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS-GROUP TRANSFERASES; PROTEINS; RODENTS; TRANSFERASES; VERTEBRATES 560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)

Citation Formats

Summers, S., Florio, T., and Cronin, M. Phorbol esters alter adenylate cyclase responses to vasoactive intestinal peptide and forskolin in the GH cell line. United States: N. p., 1986. Web.
Summers, S., Florio, T., & Cronin, M. Phorbol esters alter adenylate cyclase responses to vasoactive intestinal peptide and forskolin in the GH cell line. United States.
Summers, S., Florio, T., and Cronin, M. Thu . "Phorbol esters alter adenylate cyclase responses to vasoactive intestinal peptide and forskolin in the GH cell line". United States.
@article{osti_5325575,
title = {Phorbol esters alter adenylate cyclase responses to vasoactive intestinal peptide and forskolin in the GH cell line},
author = {Summers, S. and Florio, T. and Cronin, M.},
abstractNote = {Activation of protein kinase C with phorbol ester modifies cyclic AMP production in several anterior pituitary cell systems. In the GH cell line from a rat pituitary tumor, exposure to phorbol 12-myristate 13-acetate (PMA: 100 nM) for 30 minutes significantly reduces vasoactive intestinal peptide (VIP: 100 nM) stimulated adenylate cyclase (AC) activity in subsequent membrane preparations to 62 + 4% of control (n = 6 independent studies). In contrast, these same membrane preparations respond to forskolin (1 ..mu..M) with significantly more activity, 130 +/- 6% of controls (n = 6 independent studies). Finally, phorbol ester does not block an inhibitory hormone input into the AC system; somatostatin (100 nM) reduction of VIP-stimulated AC activity is not significantly different in membrane preparations from PMA treated and control cells (n = 3 independent studies). These other findings lead the authors to propose that protein kinase C can modify several sites in the AC complex in anterior pituitary cells.},
doi = {},
journal = {Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)},
number = ,
volume = 45:6,
place = {United States},
year = {1986},
month = {5}
}

Conference:
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