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Title: Thiophene-degrading Escherichia coli mutants possess sulfone oxidase activity and show altered resistance to sulfur-containing antibiotics

Abstract

The authors have previously isolated mutants of Escherichia coli which show increased oxidation of heterocyclic furan and thiophene substrates. They have now found that strains carrying the thdA mutation express a novel enzyme activity which oxidizes a variety of substrates containing a sulfone (e.g., ethyl sulfone) were oxidized. The thdA mutants were more resistant than wild-type strains to aromatic sulfone antibiotics such as dapsone. In contrast they showed increased susceptibility to thiolutin, a cyclic antibiotic containing sulfur at the sulfide level of oxidation. Several new thdA mutant alleles were isolated by selecting for increased oxidation of various aliphatic sulfur compounds. These new thdA mutants showed similar sulfone oxidase activity and the same map location (at 10.7 min) as the original thdA1 mutation. The constitutive fadR mutation was required for the phenotypic expression of thdA-mediated oxidation of sulfur compounds. However, the thdA-directed expression of sulfone oxidase activity was not fadR dependent. The thdC and thdD mutations probably protect against the toxicity of thiophene derivatives rather than conferring improved metabolic capability.

Authors:
;  [1]
  1. (Southern Illinois Univ., Carbondale (USA))
Publication Date:
OSTI Identifier:
5324062
DOE Contract Number:  
FG22-87PC79912
Resource Type:
Journal Article
Resource Relation:
Journal Name: Applied and Environmental Microbiology; (United States); Journal Volume: 56:10
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; OXIDOREDUCTASES; GENES; THIOPHENE; BIODEGRADATION; ANTIBIOTICS; ENZYME ACTIVITY; ESCHERICHIA COLI; MUTANTS; SULFUR COMPOUNDS; TOLERANCE; TOXICITY; ANTI-INFECTIVE AGENTS; BACTERIA; CHEMICAL REACTIONS; DECOMPOSITION; DRUGS; ENZYMES; HETEROCYCLIC COMPOUNDS; MICROORGANISMS; ORGANIC COMPOUNDS; ORGANIC SULFUR COMPOUNDS; 550200* - Biochemistry

Citation Formats

Juhl, M.J., and Clark, D.P. Thiophene-degrading Escherichia coli mutants possess sulfone oxidase activity and show altered resistance to sulfur-containing antibiotics. United States: N. p., 1990. Web.
Juhl, M.J., & Clark, D.P. Thiophene-degrading Escherichia coli mutants possess sulfone oxidase activity and show altered resistance to sulfur-containing antibiotics. United States.
Juhl, M.J., and Clark, D.P. Mon . "Thiophene-degrading Escherichia coli mutants possess sulfone oxidase activity and show altered resistance to sulfur-containing antibiotics". United States.
@article{osti_5324062,
title = {Thiophene-degrading Escherichia coli mutants possess sulfone oxidase activity and show altered resistance to sulfur-containing antibiotics},
author = {Juhl, M.J. and Clark, D.P.},
abstractNote = {The authors have previously isolated mutants of Escherichia coli which show increased oxidation of heterocyclic furan and thiophene substrates. They have now found that strains carrying the thdA mutation express a novel enzyme activity which oxidizes a variety of substrates containing a sulfone (e.g., ethyl sulfone) were oxidized. The thdA mutants were more resistant than wild-type strains to aromatic sulfone antibiotics such as dapsone. In contrast they showed increased susceptibility to thiolutin, a cyclic antibiotic containing sulfur at the sulfide level of oxidation. Several new thdA mutant alleles were isolated by selecting for increased oxidation of various aliphatic sulfur compounds. These new thdA mutants showed similar sulfone oxidase activity and the same map location (at 10.7 min) as the original thdA1 mutation. The constitutive fadR mutation was required for the phenotypic expression of thdA-mediated oxidation of sulfur compounds. However, the thdA-directed expression of sulfone oxidase activity was not fadR dependent. The thdC and thdD mutations probably protect against the toxicity of thiophene derivatives rather than conferring improved metabolic capability.},
doi = {},
journal = {Applied and Environmental Microbiology; (United States)},
number = ,
volume = 56:10,
place = {United States},
year = {Mon Oct 01 00:00:00 EDT 1990},
month = {Mon Oct 01 00:00:00 EDT 1990}
}