Cell-cell adhesion mediated by binding of membrane-anchored transforming growth factor. alpha. to epidermal growth factor receptors promotes cell proliferation

Journal Article · Tue May 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.9.3289· OSTI ID:5323188

Anklesaria, P; Greenberger, J S ^[1]; Teixido, J; Laiho, M; Massague, J ^[2]; Pierce, J H ^[3]

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1. Univ. of Massachusetts Medical Center, Worcester (USA)
2. Memorial Sloan-Kettering Cancer Center, New York, NY (USA)
3. National Cancer Institute, Bethesda, MD (USA)

The precursor for transforming growth factor {alpha}, pro-TGF-{alpha}, is a cell surface glycoprotein that can establish contact with epidermal growth factor (EGF) receptors on adjacent cells. To examine whether the pro-TGF-{alpha}/EGF receptor pair can simultaneously mediate cell adhesion and promote cell proliferation, the authors have expressed pro-TGF-{alpha} in a bone marrow stromal cell line labeled with ({sup 35}S) cysteine. Expression of pro-TGF-{alpha} allows these cells to support long-term attachment of an EGF/interleukin-3-dependent hematopoietic progenitor cell line that expresses EGF receptors but is unable to adhere to normal stroma. This interaction is inhibited by soluble EGF receptor ligands. Further, the hematopoietic progenitor cells replicate their DNA while they are attached to the stromal cell layer and become foci of sustained cell proliferation. Thus, pro-TGF-{alpha} and the EGF receptor can function as mediators of intercellular adhesion and this interaction may promote a mitogenic response. They propose the term juxtacrine to designate this form of stimulation between adjacent cells.

• OSTI ID: 5323188
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 87:9; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BONE MARROW CELLS
ADHESION
GROWTH FACTORS
RECEPTORS
CELL MEMBRANES
CELL PROLIFERATION
CYSTEINE
DNA REPLICATION
ELECTROPHORESIS
FLUORESCENCE SPECTROSCOPY
GLYCOPROTEINS
LIGANDS
SULFUR 35
AMINO ACIDS
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
EMISSION SPECTROSCOPY
EVEN-ODD NUCLEI
ISOTOPES
LIGHT NUCLEI
MEMBRANE PROTEINS
MEMBRANES
MITOGENS
NUCLEI
NUCLEIC ACID REPLICATION
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PROTEINS
RADIOISOTOPES
SOMATIC CELLS
SPECTROSCOPY
SULFUR ISOTOPES
THIOLS
550201* - Biochemistry- Tracer Techniques