Plasma membrane fatty acid-binding protein and mitochondrial glutamic-oxaloacetic transaminase of rat liver are related

Journal Article · Tue May 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.87.9.3484· OSTI ID:5323094

Berk, P D; Potter, B J; Sorrentino, D; Zhou, S L; Isola, L M; Stump, D; Kiang, C L; Thung, S ^[1]; Wada, H; Horio, Y ^[2]

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1. Mount Sinai School of Medicine, New York, NY (USA)
2. Univ. of Osaka (Japan)

The hepatic plasma membrane fatty acid-binding protein (h-FABP{sub PM}) and the mitochondrial isoenzyme of glutamic-oxaloacetic transaminase (mGOT) of rat liver have similar amino acid compositions and identical amino acid sequences for residues 3-24. Both proteins migrate with an apparent molecular mass of 43 kDa on SDS/polyacrylamide gel electrophoresis, have a similar pattern of basic charge isomers on isoelectric focusing, are eluted similarly from four different high-performance liquid chromatographic columns, have absorption maxima at 435 nm under acid conditions and 354 nm at pH 8.3, and bind oleate. Sinusoidally enriched liver plasma membranes and purified h-FABP{sub PM} have GOT enzymatic activity. Monospecific rabbit antiserum against h-FABP{sub PM} reacts on Western blotting with mGOT, and vice versa. Antisera against both proteins produce plasma membrane immunofluorescence in rat hepatocytes and selectively inhibit the hepatocellular uptake of ({sup 3}H)oleate but not that of ({sup 35}S)sulfobromophthalein or ({sup 14}C)taurocholate. The inhibition of oleate uptake produced by anti-h-FABP{sub PM} can be eliminated by preincubation of the antiserum with mGOT; similarly, the plasma membrane immunofluorescence produced by either antiserum can be eliminated by preincubation with the other antigen. These data suggest that h-FABP{sub PM} and mGOT are closely related.

• OSTI ID: 5323094
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 87:9; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMINOTRANSFERASES
BIOCHEMICAL REACTION KINETICS
LABELLED COMPOUNDS
UPTAKE
PROTEINS
AMINO ACID SEQUENCE
CARBON 14 COMPOUNDS
CARBOXYLIC ACIDS
CELL MEMBRANES
CHOLIC ACID
DOSE-RESPONSE RELATIONSHIPS
ELECTROPHORESIS
ISOENZYMES
LIQUID COLUMN CHROMATOGRAPHY
MITOCHONDRIA
OLEIC ACID
PHTHALIC ACID
RABBITS
SULFUR 35
TAURINE
TRITIUM COMPOUNDS
AMINES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BILE ACIDS
CARBON COMPOUNDS
CELL CONSTITUENTS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DICARBOXYLIC ACIDS
ENZYMES
EVEN-ODD NUCLEI
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPES
KINETICS
LIGHT NUCLEI
MAMMALS
MEMBRANES
MOLECULAR STRUCTURE
MONOCARBOXYLIC ACIDS
NITROGEN TRANSFERASES
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
STEROIDS
STEROLS
SULFONIC ACIDS
SULFUR ISOTOPES
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques