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Title: Intestinal mucosal mast cells from rats infected with Nippostrongylus brasiliensis contain protease-resistant chondroitin sulfate di-B proteoglycans

Abstract

Rats infected with the helminth Nippostrongylus brasiliensis were injected i.p. with 2 mCi of (/sup 35/S) sulfate on days 13, 15, 17, and 19 after infection. The intestines were removed from animals on day 20 or 21 after infection, the intestinal cells were obtained by collagenase treatment and mechanical dispersion of the tissue, and the /sup 35/S-labeled mucosal mast cells (MMC) were enriched to 60 to 65% purity by Percoll centrifugation. The isolated proteoglycans were of approx. 150,000 m.w., were resistant to pronase degradation, and contained highly sulfated chondroitin sulfate side chains. The presence in normal mammalian cells of chondroitin sulfate proteoglycans that contain a high percentage of the unusual disulfated di-B disaccharide has not been previously reported. The rat intestinal MMC proteoglycans are the first chondroitin sulfate proteoglycans that have been isolated from an enriched populations of normal mast cells. They are homologous to the chondroitin sulfate-rich proteoglycans of the transformed rat basophilic leumekia-1 cell and the cultured interleukin 3-dependent mouse bone marrow-derived mast cell, in that these chondroitin sulfate proteoglycans are all highly sulfated, protease-resistant proteoglycans.

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Harvard Medical School, Boston, MA
OSTI Identifier:
5322945
Resource Type:
Journal Article
Journal Name:
J. Immunol.; (United States)
Additional Journal Information:
Journal Volume: 137:1
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GLUCOPROTEINS; BIOCHEMICAL REACTION KINETICS; HELMINTHS; INFECTIVITY; MAST CELLS; MOLECULAR BIOLOGY; ANIMAL CELLS; CHONDROITIN; INTESTINES; MUCOUS MEMBRANES; SULFATES; SULFUR 35; TRACER TECHNIQUES; AMINES; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARBOHYDRATES; CONNECTIVE TISSUE CELLS; DAYS LIVING RADIOISOTOPES; DIGESTIVE SYSTEM; EVEN-ODD NUCLEI; GASTROINTESTINAL TRACT; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LIGHT NUCLEI; MEMBRANES; MUCOPOLYSACCHARIDES; NUCLEI; ORGANIC COMPOUNDS; ORGANS; OXYGEN COMPOUNDS; POLYSACCHARIDES; PROTEINS; RADIOISOTOPES; REACTION KINETICS; SACCHARIDES; SOMATIC CELLS; SULFUR COMPOUNDS; SULFUR ISOTOPES; 550201* - Biochemistry- Tracer Techniques; 550301 - Cytology- Tracer Techniques

Citation Formats

Stevens, R L, Lee, T D.G., Seldin, D C, Austen, K F, Befus, A D, and Bienenstock, J. Intestinal mucosal mast cells from rats infected with Nippostrongylus brasiliensis contain protease-resistant chondroitin sulfate di-B proteoglycans. United States: N. p., 1986. Web.
Stevens, R L, Lee, T D.G., Seldin, D C, Austen, K F, Befus, A D, & Bienenstock, J. Intestinal mucosal mast cells from rats infected with Nippostrongylus brasiliensis contain protease-resistant chondroitin sulfate di-B proteoglycans. United States.
Stevens, R L, Lee, T D.G., Seldin, D C, Austen, K F, Befus, A D, and Bienenstock, J. 1986. "Intestinal mucosal mast cells from rats infected with Nippostrongylus brasiliensis contain protease-resistant chondroitin sulfate di-B proteoglycans". United States.
@article{osti_5322945,
title = {Intestinal mucosal mast cells from rats infected with Nippostrongylus brasiliensis contain protease-resistant chondroitin sulfate di-B proteoglycans},
author = {Stevens, R L and Lee, T D.G. and Seldin, D C and Austen, K F and Befus, A D and Bienenstock, J},
abstractNote = {Rats infected with the helminth Nippostrongylus brasiliensis were injected i.p. with 2 mCi of (/sup 35/S) sulfate on days 13, 15, 17, and 19 after infection. The intestines were removed from animals on day 20 or 21 after infection, the intestinal cells were obtained by collagenase treatment and mechanical dispersion of the tissue, and the /sup 35/S-labeled mucosal mast cells (MMC) were enriched to 60 to 65% purity by Percoll centrifugation. The isolated proteoglycans were of approx. 150,000 m.w., were resistant to pronase degradation, and contained highly sulfated chondroitin sulfate side chains. The presence in normal mammalian cells of chondroitin sulfate proteoglycans that contain a high percentage of the unusual disulfated di-B disaccharide has not been previously reported. The rat intestinal MMC proteoglycans are the first chondroitin sulfate proteoglycans that have been isolated from an enriched populations of normal mast cells. They are homologous to the chondroitin sulfate-rich proteoglycans of the transformed rat basophilic leumekia-1 cell and the cultured interleukin 3-dependent mouse bone marrow-derived mast cell, in that these chondroitin sulfate proteoglycans are all highly sulfated, protease-resistant proteoglycans.},
doi = {},
url = {https://www.osti.gov/biblio/5322945}, journal = {J. Immunol.; (United States)},
number = ,
volume = 137:1,
place = {United States},
year = {Tue Jul 01 00:00:00 EDT 1986},
month = {Tue Jul 01 00:00:00 EDT 1986}
}