Manganese regulation of manganese peroxidase expression and lignin degradation by the white rot fungus Dichomitus squalens
- Oregon Graduate Institute of Science and Technology, Beaverton (USA)
Extracellular manganese peroxidase and laccase activities were detected in cultures of Dichomitus squalens (Polyporus anceps) under conditions favoring lignin degradation. In contrast, neither extracellular lignin peroxidase nor aryl alcohol oxidase activity was detected in cultures grown under a wide variety of conditions. The mineralization of {sup 14}C-ring-, -side chain-, and -methoxy-labeled synthetic guaiacyl lignins by D. squalens and the expression of extracellular manganese peroxidase were dependent on the presence of Mn(II), suggesting that manganese peroxidase is an important component of this organism's lignin degradation system. The expression of laccase activity was independent of manganese. In contrast to previous findings with Phanero-chaete chrysosporium, lignin degradation by D. squalens proceeded in the cultures containing excess carbon and nitrogen.
- DOE Contract Number:
- FG06-87ER13715
- OSTI ID:
- 5319660
- Journal Information:
- Applied and Environmental Microbiology; (United States), Vol. 57:8; ISSN 0099-2240
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
LIGNIN
BIODEGRADATION
PEROXIDASES
ENZYME INDUCTION
CARBON 14 COMPOUNDS
ENZYME ACTIVITY
FUNGI
MANGANESE
NITROGEN
TRACER TECHNIQUES
CARBOHYDRATES
CARBON COMPOUNDS
CHEMICAL REACTIONS
DECOMPOSITION
ELEMENTS
ENZYMES
GENE REGULATION
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METALS
NONMETALS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
PLANTS
POLYSACCHARIDES
SACCHARIDES
TRANSITION ELEMENTS
540210* - Environment
Terrestrial- Basic Studies- (1990-)
090900 - Biomass Fuels- Processing- (1990-)
550501 - Metabolism- Tracer Techniques