Synthesis and use of radio cobaltic EDTA as an extracellular marker in rabbit heart

Journal Article · Thu Apr 01 00:00:00 EST 1982 · Am. J. Physiol.; (United States)

OSTI ID:5313819

Bridge, J H.B. ^[1]; Bersohn, M M; Gonzalez, F; Bassingthwaighte, J B

---

1. Univ. of Washington, Seattle

A new ..gamma..-labeled marker for extracellular space is the cobaltic form of /sup 58/Co-ethylenediaminetetraacetic acid (/sup 58/Co-EDTA). The cobaltic ion has a much higher affinity for EDTA than the cobaltous ion; it is prepared as a potassium salt, K/sup +/(/sup 58/Co/sup 3 +/-EDTA/sup 4 -/), and is apparently biologically inert. Testing by equilibrium in intact rabbits and comparing the myocardial content with that of (/sup 14/C)sucrose give values of the volume of distribution in the myocardium of 0.294 +/- 0.052 ml/g for /sup 58/Co-EDTA and 0.303 +/- 0.051 ml/g for (/sup 14/C)sucrose (SD, n = 130, for two hearts), with the ratios of /sup 58/Co-EDTA/sucrose averaging 0.973 +/- 0.043 (n = 130). The average value of the extracellular fluid measured in isolated rabbit interventricular septum using Co-EDTA was 0.51 +/- 0.05 ml/g (SD, n = 16) and 0.46 +/- 0.04 ml/g using (/sup 14/C)sucrose as an extracellular fluid space (ECF) marker. Flushing with a high concentration of nontracer Co-EDTA does not reveal any release from binding sites. The ..gamma..-energy (811 KeV), long half-life (71.4 days), stability, and lack of binding to tissue components make /sup 58/Co-EDTA a useful marker for ECF.

• OSTI ID: 5313819
• Journal Information: Am. J. Physiol.; (United States), Vol. 242:4
• Country of Publication: United States
• Language: English

Related Subjects

38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
62 RADIOLOGY AND NUCLEAR MEDICINE
COBALT 58
TISSUE DISTRIBUTION
COBALT COMPLEXES
LABELLING
EDTA
EXTRACELLULAR SPACE
TRACER TECHNIQUES
BIOLOGICAL LOCALIZATION
CHEMICAL PREPARATION
GAMMA RADIATION
HEART
LABELLED COMPOUNDS
RABBITS
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHELATING AGENTS
COBALT ISOTOPES
COMPLEXES
DAYS LIVING RADIOISOTOPES
DISTRIBUTION
ELECTROMAGNETIC RADIATION
ELECTRON CAPTURE RADIOISOTOPES
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RADIATIONS
RADIOISOTOPES
SPACE
SYNTHESIS
TRANSITION ELEMENT COMPLEXES
VERTEBRATES
400702* - Radiochemistry & Nuclear Chemistry- Properties of Radioactive Materials
550601 - Medicine- Unsealed Radionuclides in Diagnostics