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Title: Determination of the distribution of selenium between selenoprotein P, glutathione peroxidase and albumin in plasma

Abstract

A chromatographic method was developed to determine the distribution of selenium between selenoprotein P, glutathione peroxidase (GSH-Px) and albumin, using two small columns of heparin-Sepharose CL-6B (white) and Reactive Blue 2-Sepharose CL-6B (blue) linked together in tandem. One ml of plasma was diluted to 10 ml with 0.02 M sodium phosphate buffer, pH 7.0, the equilibration buffer, and applied to the white column. This was eluted at flow rate of 30 ml per hour. GSHp-Px was not retained by either column but selenoprotein P was retained by the white column and albumin by the blue column. After the two columns were separated, selenoprotein P was eluted for 90 min from the white column with a solution containing 500 units of heparin per ml. The albumin was eluted for 55 min from the blue column with 1.4 M NaCl. This method indicated that over 50% of the selenium in plasma from rats, monkeys, humans and sheep was with selenoprotein P, even during deficiency. From 15 to 22% of the selenium was associated with GSH-Px. The percentage of selenium with albumin was dependent upon the selenomethionine content of the diet.

Authors:
; ;  [1]
  1. Oregon State Univ., Corvallis (United States)
Publication Date:
OSTI Identifier:
5300286
Report Number(s):
CONF-9104107-
Journal ID: ISSN 0892-6638; CODEN: FAJOE
Resource Type:
Conference
Journal Name:
FASEB Journal (Federation of American Societies for Experimental Biology); (United States)
Additional Journal Information:
Journal Volume: 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; Journal ID: ISSN 0892-6638
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; METALLOPROTEINS; BIOSYNTHESIS; SELENIUM; METABOLISM; ALBUMINS; BLOOD PLASMA; GLUTATHIONE; MAN; MONKEYS; NUTRITIONAL DEFICIENCY; PEROXIDASES; RATS; SHEEP; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; DOMESTIC ANIMALS; DRUGS; ELEMENTS; ENZYMES; MAMMALS; MATERIALS; ORGANIC COMPOUNDS; OXIDOREDUCTASES; PEPTIDES; POLYPEPTIDES; PRIMATES; PROTEINS; RADIOPROTECTIVE SUBSTANCES; RODENTS; RUMINANTS; SEMIMETALS; SYNTHESIS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Whanger, P D, Butler, J A, and Deagen, J T. Determination of the distribution of selenium between selenoprotein P, glutathione peroxidase and albumin in plasma. United States: N. p., 1991. Web.
Whanger, P D, Butler, J A, & Deagen, J T. Determination of the distribution of selenium between selenoprotein P, glutathione peroxidase and albumin in plasma. United States.
Whanger, P D, Butler, J A, and Deagen, J T. 1991. "Determination of the distribution of selenium between selenoprotein P, glutathione peroxidase and albumin in plasma". United States.
@article{osti_5300286,
title = {Determination of the distribution of selenium between selenoprotein P, glutathione peroxidase and albumin in plasma},
author = {Whanger, P D and Butler, J A and Deagen, J T},
abstractNote = {A chromatographic method was developed to determine the distribution of selenium between selenoprotein P, glutathione peroxidase (GSH-Px) and albumin, using two small columns of heparin-Sepharose CL-6B (white) and Reactive Blue 2-Sepharose CL-6B (blue) linked together in tandem. One ml of plasma was diluted to 10 ml with 0.02 M sodium phosphate buffer, pH 7.0, the equilibration buffer, and applied to the white column. This was eluted at flow rate of 30 ml per hour. GSHp-Px was not retained by either column but selenoprotein P was retained by the white column and albumin by the blue column. After the two columns were separated, selenoprotein P was eluted for 90 min from the white column with a solution containing 500 units of heparin per ml. The albumin was eluted for 55 min from the blue column with 1.4 M NaCl. This method indicated that over 50% of the selenium in plasma from rats, monkeys, humans and sheep was with selenoprotein P, even during deficiency. From 15 to 22% of the selenium was associated with GSH-Px. The percentage of selenium with albumin was dependent upon the selenomethionine content of the diet.},
doi = {},
url = {https://www.osti.gov/biblio/5300286}, journal = {FASEB Journal (Federation of American Societies for Experimental Biology); (United States)},
issn = {0892-6638},
number = ,
volume = 5:4,
place = {United States},
year = {Mon Mar 11 00:00:00 EST 1991},
month = {Mon Mar 11 00:00:00 EST 1991}
}

Conference:
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