Phorbol esters modulate the shape of cultured canine vascular smooth muscle cells
- Univ. of Minnesota, Duluth (United States)
Marked changes in the shape of vascular smooth muscle cells (VSMC) occur during early development, repair of the vascular wall, and formation of atherosclerotic plaques. Yet, surprisingly little is known about mechanisms which regulate the shape of VSMC. Since protein kinase C (PKC) is involved in regulation of multiple cellular functions including interactions between contractile and cytoskeletal proteins, the authors suspected it might also regulate VSMC shape. Accordingly, the authors studied the influence of a known activator of PKC, phorbol 12-myristate 13-acetate (PMA), on the shape of cultured canine carotid arterial BSMC. PMA produced time and concentration dependent changes from normal elongated shape to pronounced circular forms. Cells recovered normal shape within 24 hrs even though exposure to PMA was continued. Analogs of PMA which do not activate PKC did not alter shape, whereas phorbol 13, 14 diacetate, an analog which activates PKC, did produce changes in shape similar to those produced by PMA. Cycloheximide, an inhibitor of protein synthesis, or actinomycin D, an inhibitor of mRNA synthesis, did not alter PMA-induced changes in morphology. In contrast, however, recovery of normal shape after prolonged exposure to PMA was blocked by either cycloheximide or actinomycin D. These results suggest activation of PKC produces changes in VSMC shape that are independent of transcription or translation, whereas recovery is dependent on both transcription and translation. The results also suggest PKC may modulate in vivo changes in VSMC shape occurring during different pathophysiological states.
- OSTI ID:
- 5300081
- Report Number(s):
- CONF-9104107-; CODEN: FAJOE
- Journal Information:
- FASEB Journal (Federation of American Societies for Experimental Biology); (United States), Vol. 5:4; Conference: 75. annual meeting of the Federation of American Societies for Experimental Biology (FASEB), Atlanta, GA (United States), 21-25 Apr 1991; ISSN 0892-6638
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANIMAL CELLS
MORPHOLOGY
ARTERIOSCLEROSIS
PATHOGENESIS
PHORBOL ESTERS
BIOLOGICAL EFFECTS
ACTINOMYCIN
CAROTID ARTERIES
CELL CULTURES
CYCLOHEXIMIDE
DOGS
DOSE-RESPONSE RELATIONSHIPS
MESSENGER-RNA
MUSCLES
TIME DEPENDENCE
TRANSCRIPTION
ANIMALS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
ARTERIES
BLOOD VESSELS
BODY
CARCINOGENS
CARDIOVASCULAR DISEASES
CARDIOVASCULAR SYSTEM
DISEASES
DRUGS
ESTERS
FUNGICIDES
MAMMALS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PESTICIDES
RNA
VASCULAR DISEASES
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology