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Title: Comparison of effects of ATP-MgCl/sub 2/ and adenosine-MgCl/sub 2/ on renal function following ischemia

Abstract

ATO-MgCl/sub 2/ administration had been shown to accelerate the recovery of renal function following warm ischemia. However, since the major breakdown product of ATP is adenosine, the relative contribution of ATP vs. adenosine in improving renal function following ischemia remains to be determined. To study this, kidneys were subjected to 45 min of normothermic ischemia and then perfused at 100 mmHg with oxygenated Krebs-HCO/sub 3/ buffer containing albumin, (/sub 3/H)inulin, substrates, and either 0.3 mM ATP-MgCl/sub 2/ or adenosine-MgCl/sub 2/ for 110 min. Perfusate and timed urine samples were collected and analyzed for radioactivity and (Na/sup +/). The functional parameters indicated that although adenosine-MgCl/sub 2/ treatment provided a transient improvement, it failed to provided a sustained improvement in renal function or attain control valued compared with ATP-MgCl/sub 2/ treatment. Thus, the salutary effects of ATP-MgCl/sub 2/ following warm ischemia in the kidney are not mediated by adenosine.

Authors:
; ; ;
Publication Date:
Research Org.:
Yale Univ. School of Medicine, New Haven, CT
OSTI Identifier:
5298913
Resource Type:
Journal Article
Journal Name:
Am. J. Physiol.; (United States)
Additional Journal Information:
Journal Volume: 252:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ADENOSINE; BIOCHEMISTRY; ATP; KIDNEYS; PHYSIOLOGY; TRITIUM COMPOUNDS; TRACER TECHNIQUES; INULIN; ISCHEMIA; MAGNESIUM CHLORIDES; URINE; ALKALINE EARTH METAL COMPOUNDS; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; BODY; BODY FLUIDS; CARBOHYDRATES; CARDIOVASCULAR DISEASES; CHEMISTRY; CHLORIDES; CHLORINE COMPOUNDS; DISEASES; HALIDES; HALOGEN COMPOUNDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAGNESIUM COMPOUNDS; MATERIALS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANS; POLYSACCHARIDES; RIBOSIDES; SACCHARIDES; VASCULAR DISEASES; WASTES; 551001* - Physiological Systems- Tracer Techniques

Citation Formats

Sumpio, B E, Hull, M J, Baue, A E, and Chaudry, I H. Comparison of effects of ATP-MgCl/sub 2/ and adenosine-MgCl/sub 2/ on renal function following ischemia. United States: N. p., 1987. Web.
Sumpio, B E, Hull, M J, Baue, A E, & Chaudry, I H. Comparison of effects of ATP-MgCl/sub 2/ and adenosine-MgCl/sub 2/ on renal function following ischemia. United States.
Sumpio, B E, Hull, M J, Baue, A E, and Chaudry, I H. 1987. "Comparison of effects of ATP-MgCl/sub 2/ and adenosine-MgCl/sub 2/ on renal function following ischemia". United States.
@article{osti_5298913,
title = {Comparison of effects of ATP-MgCl/sub 2/ and adenosine-MgCl/sub 2/ on renal function following ischemia},
author = {Sumpio, B E and Hull, M J and Baue, A E and Chaudry, I H},
abstractNote = {ATO-MgCl/sub 2/ administration had been shown to accelerate the recovery of renal function following warm ischemia. However, since the major breakdown product of ATP is adenosine, the relative contribution of ATP vs. adenosine in improving renal function following ischemia remains to be determined. To study this, kidneys were subjected to 45 min of normothermic ischemia and then perfused at 100 mmHg with oxygenated Krebs-HCO/sub 3/ buffer containing albumin, (/sub 3/H)inulin, substrates, and either 0.3 mM ATP-MgCl/sub 2/ or adenosine-MgCl/sub 2/ for 110 min. Perfusate and timed urine samples were collected and analyzed for radioactivity and (Na/sup +/). The functional parameters indicated that although adenosine-MgCl/sub 2/ treatment provided a transient improvement, it failed to provided a sustained improvement in renal function or attain control valued compared with ATP-MgCl/sub 2/ treatment. Thus, the salutary effects of ATP-MgCl/sub 2/ following warm ischemia in the kidney are not mediated by adenosine.},
doi = {},
url = {https://www.osti.gov/biblio/5298913}, journal = {Am. J. Physiol.; (United States)},
number = ,
volume = 252:2,
place = {United States},
year = {Sun Feb 01 00:00:00 EST 1987},
month = {Sun Feb 01 00:00:00 EST 1987}
}