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Title: Partial acid hydrolysis of poplar wood as a pretreatment for enzymatic hydrolysis

Abstract

Partial acid hydrolysis was studied as a pretreatment to enhance glucose yields from enzymatic hydrolysis of poplar. The pretreatments were carried out in a continuous flow reactor at temperatures ranging from 162 to 222/sup 0/C, acid concentrations ranging from 0 to 1.5%, and treatment times from 3.6 to 12.7 s. The pretreated slurries were hydrolyzed with Trichoderma reesei C30 cellulase at 50/sup 0/C and a pH of 4.8 for 48 h. Increased yields of glucose were achieved when poplar was pretreated at temperatures higher than 180/sup 0/C. By increasing the cellobiase activity of the cellulase with the addition of NOVO cellobiase, in some cases 100% of the potential glucose content of the substrate was converted to glucose after only 24 h of enzymatic hydrolysis.

Authors:
; ;
Publication Date:
Research Org.:
Dartmouth Coll., Hanover, NH
OSTI Identifier:
5287445
Report Number(s):
CONF-810554-
Journal ID: CODEN: BIBSB
Resource Type:
Conference
Journal Name:
Biotechnol. Bioeng. Symp.; (United States)
Additional Journal Information:
Journal Volume: 11; Conference: 3. symposium on biotechnology in energy production and conservation, Gatlinburg, TN, USA, 12 May 1981
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; WOOD; ACID HYDROLYSIS; ENZYMATIC HYDROLYSIS; CELLULASE; CELLULOSE; GLUCOSE; POPLARS; SACCHARIFICATION; ALDEHYDES; CARBOHYDRATES; CHEMICAL REACTIONS; DECOMPOSITION; ENZYMES; GLYCOSYL HYDROLASES; HEXOSES; HYDROLASES; HYDROLYSIS; LYSIS; MONOSACCHARIDES; O-GLYCOSYL HYDROLASES; ORGANIC COMPOUNDS; PLANTS; POLYSACCHARIDES; SACCHARIDES; SOLVOLYSIS; TREES; 140504* - Solar Energy Conversion- Biomass Production & Conversion- (-1989); 090222 - Alcohol Fuels- Preparation from Wastes or Biomass- (1976-1989)

Citation Formats

Knappert, D, Grethlein, H, and Converse, A. Partial acid hydrolysis of poplar wood as a pretreatment for enzymatic hydrolysis. United States: N. p., 1981. Web.
Knappert, D, Grethlein, H, & Converse, A. Partial acid hydrolysis of poplar wood as a pretreatment for enzymatic hydrolysis. United States.
Knappert, D, Grethlein, H, and Converse, A. Thu . "Partial acid hydrolysis of poplar wood as a pretreatment for enzymatic hydrolysis". United States.
@article{osti_5287445,
title = {Partial acid hydrolysis of poplar wood as a pretreatment for enzymatic hydrolysis},
author = {Knappert, D and Grethlein, H and Converse, A},
abstractNote = {Partial acid hydrolysis was studied as a pretreatment to enhance glucose yields from enzymatic hydrolysis of poplar. The pretreatments were carried out in a continuous flow reactor at temperatures ranging from 162 to 222/sup 0/C, acid concentrations ranging from 0 to 1.5%, and treatment times from 3.6 to 12.7 s. The pretreated slurries were hydrolyzed with Trichoderma reesei C30 cellulase at 50/sup 0/C and a pH of 4.8 for 48 h. Increased yields of glucose were achieved when poplar was pretreated at temperatures higher than 180/sup 0/C. By increasing the cellobiase activity of the cellulase with the addition of NOVO cellobiase, in some cases 100% of the potential glucose content of the substrate was converted to glucose after only 24 h of enzymatic hydrolysis.},
doi = {},
url = {https://www.osti.gov/biblio/5287445}, journal = {Biotechnol. Bioeng. Symp.; (United States)},
number = ,
volume = 11,
place = {United States},
year = {1981},
month = {1}
}

Conference:
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