Partial acid hydrolysis of poplar wood as a pretreatment for enzymatic hydrolysis
Partial acid hydrolysis was studied as a pretreatment to enhance glucose yields from enzymatic hydrolysis of poplar. The pretreatments were carried out in a continuous flow reactor at temperatures ranging from 162 to 222/sup 0/C, acid concentrations ranging from 0 to 1.5%, and treatment times from 3.6 to 12.7 s. The pretreated slurries were hydrolyzed with Trichoderma reesei C30 cellulase at 50/sup 0/C and a pH of 4.8 for 48 h. Increased yields of glucose were achieved when poplar was pretreated at temperatures higher than 180/sup 0/C. By increasing the cellobiase activity of the cellulase with the addition of NOVO cellobiase, in some cases 100% of the potential glucose content of the substrate was converted to glucose after only 24 h of enzymatic hydrolysis.
- Research Organization:
- Dartmouth Coll., Hanover, NH
- OSTI ID:
- 5287445
- Report Number(s):
- CONF-810554-
- Journal Information:
- Biotechnol. Bioeng. Symp.; (United States), Vol. 11; Conference: 3. symposium on biotechnology in energy production and conservation, Gatlinburg, TN, USA, 12 May 1981
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
WOOD
ACID HYDROLYSIS
ENZYMATIC HYDROLYSIS
CELLULASE
CELLULOSE
GLUCOSE
POPLARS
SACCHARIFICATION
ALDEHYDES
CARBOHYDRATES
CHEMICAL REACTIONS
DECOMPOSITION
ENZYMES
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
TREES
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