Microfilter paper method for 17. cap alpha. -hydroxyprogesterone radioimmunoassay: its application for rapid screening for congenital adrenal hyperplasia. [Tritium tracer techniques]

Journal Article · Tue Nov 01 00:00:00 EST 1977 · J. Clin. Endocrinol. Metab.; (United States)

DOI:https://doi.org/10.1210/jcem-45-5-1003· OSTI ID:5276711

Pang, S; Hotchkiss, J; Drash, A L; Levine, L S; New, M I

A new micromethod for measuring a steroid in blood collected on filter paper has been developed. The method is easy and rapid and has the specificity, accuracy and precision of RIA in whole plasma. Less than 20 ..mu..l of blood is required, and, therefore, samples may be obtained with heel prick. This method has been applied to the determination of 17..cap alpha..-hydroxyprogesterone (17..cap alpha..-OH-P) for screening patients with congenital adrenal hyperplasia (CAH) due to 21-hydroxylase deficiency. There was excellent correlation (r = .94) between the values of 17..cap alpha..-OH-P obtained by microfilter paper method and those from plasma samples of cord (40 +- 13 ng/ml) and neonatal blood (<3.6 ng/ml) in normal infants. In six neonates at risk for CAH the diagnosis was made utilizing the microfilter paper method. 17..cap alpha..-OH-P concentrations were highly elevated in both filter paper eluates of whole blood (67 to 360 ng/ml of plasma) and simultaneously obtained plasma concentration (74 to 395 ng/ml) in affected infants. The concentrations of 17..cap alpha..-OH-P remained unchanged in dried filter paper blood when stored at room temperature for up to 21 days. Thus, filter paper with dried blood may be sent for steroid assay by mail. The ease with which samples may be transported and the minute amount of sample necessary make this method a promising screening test for CAH.

• Research Organization: New York Hospital-Cornell Medical Center, New York
• OSTI ID: 5276711
• Journal Information: J. Clin. Endocrinol. Metab.; (United States), Vol. 45:5
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
PROGESTERONE
RADIOIMMUNOASSAY
ADRENAL GLANDS
BLOOD
INFANTS
NEONATES
PATIENTS
TRACER TECHNIQUES
TRITIUM
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CHILDREN
ENDOCRINE GLANDS
GLANDS
HORMONES
HYDROGEN ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KETONES
LIGHT NUCLEI
MAMMALS
MAN
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PREGNANES
PRIMATES
RADIOASSAY
RADIOISOTOPES
STEROID HORMONES
STEROIDS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
550601* - Medicine- Unsealed Radionuclides in Diagnostics