Liquefaction of coal by Polyporus versicolor and Poria monticola. Final report, 1 September 1984-31 August 1986

Technical Report · Wed Jan 01 00:00:00 EST 1986

OSTI ID:5270252

Cohen, M S

Polyporus versicolor (ATCC 12679), obtained from the American Type Culture Collection, Rockville, MD, has been demonstrated to degrade leonardite, lignite, and subbituminous coals to a black liquid product which is called the bioextract. The process of solubilizing the coal has been termed liquification. Fungi were routinely maintained in both solid Sabouraud maltose agar (6%) and in Sabouraud maltose broth cultures. All cultures were incubated at 30/sup 0/C, 84 to 98% relative humidity, and pH = 5.8. All materials which came into contact with the fungi were sterilized before use. Experimental cultures were incubated as described for stock cultures. Cultures were incubated for approximately 12 days to produce a mature fungal mat across a glass petri dish. Coal pieces (approximately 5 mm/sup 3/) were placed directly on the hyphal mat. Liquified coal (the bioextract) was removed from the top of the mycelium and/or coal pieces and either stored for analyses at 4/sup 0/C or else freeze-dried and stored dessicated at room temperature. The bioextract has been produced in sufficient quantity to permit various methods of analysis including high performance liquid chromatography, UV-visible spectrophotometry, titrimetry, electrophoresis, proton nmr spectroscopy, and calorimetry. The solubility of the bioextract in different solvents has also been determined. 6 refs., 26 figs., 3 tabs.

• Research Organization: Hartford Univ., West Hartford, CT (USA). Dept. of Biology and Health Science
• DOE Contract Number: FG22-84PC70806
• OSTI ID: 5270252
• Report Number(s): DOE/PC/70806-T4; ON: DE86014997
• Resource Relation: Other Information: Portions of this document are illegible in microfiche products
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
COAL LIQUEFACTION
LIGNITE
BIODEGRADATION
COAL LIQUIDS
CULTURE MEDIA
ELECTROPHORESIS
EXPERIMENTAL DATA
FUNGI
HYDROGEN 1
LIQUID COLUMN CHROMATOGRAPHY
NUCLEAR MAGNETIC RESONANCE
RESEARCH PROGRAMS
SPECTROPHOTOMETRY
TITRATION
BROWN COAL
CARBONACEOUS MATERIALS
CHEMICAL REACTIONS
CHROMATOGRAPHY
COAL
DATA
DECOMPOSITION
ENERGY SOURCES
FLUIDS
FOSSIL FUELS
FUELS
HYDROGEN ISOTOPES
INFORMATION
ISOTOPES
LIGHT NUCLEI
LIQUEFACTION
LIQUIDS
MAGNETIC RESONANCE
MATERIALS
NUCLEI
NUMERICAL DATA
ODD-EVEN NUCLEI
PLANTS
RESONANCE
SEPARATION PROCESSES
STABLE ISOTOPES
THERMOCHEMICAL PROCESSES
010405* - Coal
Lignite
& Peat- Hydrogenation & Liquefaction