Semiquantitative probe for radiation-induced normal tissue damage at the molecular level

Journal Article · Wed Jan 01 00:00:00 EST 1986 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576727· OSTI ID:5268868

Miller, G; Siemann, D; Scott, P; Dawson, D; Muldrew, K; Trepanier, P; McGann, L

Sheep antibodies to bovine type I collagen were employed in the immunohistochemical detection of type I collagen in lung tissue sections of irradiated LAF1 mice. A video image digitizing system was developed to estimate collagen levels, by assigning a numerical value (0-63) to each of approximately 53,800 picture elements (pixels) in the microscope field, according to the collagen-dependent fluorescence intensity at each locus. For lungs harvested 52 weeks subsequent to graded doses of 60Co gamma radiation between 0 and 10 Gy, a dose-dependent increase in type I collagen was observed in the alveolar walls. A reproducible increase was evident for doses as low as 5 Gy: doses of 7 to 10 Gy elicited type I collagen levels significantly elevated with respect to those of age-matched controls. These results are consistent with a role for type I collagen in the development of radiation-induced pulmonary fibrosis. The assay system developed here will be used to explore the role of connective tissue macromolecules in the development of radiation pneumonitis and fibrosis.

• Research Organization: Cross Cancer Institute, Edmonton, Alberta
• OSTI ID: 5268868
• Journal Information: Radiat. Res.; (United States), Vol. 1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
COLLAGEN
HISTOLOGICAL TECHNIQUES
LUNGS
BIOLOGICAL RADIATION EFFECTS
ANTIBODIES
COBALT 60
GAMMA RADIATION
MICE
RADIATION INJURIES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BODY
COBALT ISOTOPES
ELECTROMAGNETIC RADIATION
INJURIES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MAMMALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
SCLEROPROTEINS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560152* - Radiation Effects on Animals- Animals