Post transcriptional regulation of chloroplast gene expression by nuclear encoded gene products
The following is a review of research accomplished in the first two years of funding for the above mentioned project. The work performed is a molecular characterization of nuclear mutants of Chlamydomonas reinhardtii which are deficient in different stages in the post-transcriptional expression of a single chloroplast encoded polypeptide, the D2 protein of Photosystem II. Our long-term goals are to understand the molecular mechanisms by which nuclear gene products affect the expression of chloroplast genes. Specifically, we which to understand how specific nuclear gene products affect the turnover rate of the D2 encoding mRNA (psbD), how other nuclear encoded factors work to promote the translation of psbD mRNA and/or stabilize the D2 protein, and what the role of the D2 protein itself is in Photosystem II assembly and in the control of expression of other chloroplast genes. This progress report will be organized into four major sections concerning (I) The characterization of nuclear mutants affected in D2 translation/turnover, (II) The study of trans-acting factors which associate with the 5{prime} end of the psbD mRNA, (III) In vitro mutagenesis of the psbD gene, and (IV) Additional studies.
- Research Organization:
- Lehigh Univ., Bethlehem, PA (United States)
- Sponsoring Organization:
- USDOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- FG02-90ER20009
- OSTI ID:
- 5268747
- Report Number(s):
- DOE/ER/20009-1; ON: DE92013486
- Country of Publication:
- United States
- Language:
- English
Similar Records
Post transcriptional regulation of chloroplast gene expression by nuclear encoded gene products. Progress report, June 1, 1990--June 30, 1992
Post transcriptional regulation of chloroplast gene expression by nuclear encoded gene products
Related Subjects
CHLAMYDOMONAS
CHLOROPLASTS
GENE REGULATION
GENE REPRESSORS
MUTANTS
PHOTOSYNTHESIS
PROGRESS REPORT
PROTEINS
TRANSCRIPTION
ALGAE
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHLOROPHYCOTA
DOCUMENT TYPES
MICROORGANISMS
ORGANIC COMPOUNDS
PHOTOCHEMICAL REACTIONS
PLANTS
SYNTHESIS
UNICELLULAR ALGAE
550400* - Genetics